SBB13Ntbk-ChristinaS: Difference between revisions
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PCA quiC-4 - oligo1-16 (464bp, pca1) | PCA quiC-4 - oligo1-16 (464bp, pca1) | ||
Revision as of 09:43, 12 March 2013
PCA quiC-4 - oligo1-16 (464bp, pca1) PCR pca1 with quiC-NewForward/Reverse (418bp, pca2) Digest pca2 (EcoRI/BglII, 402+10+6,L, pcrdig) Digest pBca9145-Bca144 (EcoRI/BglII, 2958+9, L, vectdig) Ligate pcrdig and vectdig (pBca9145-quiC4) ------------------------- >oligo1 AAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTAC >oligo2 AACTTTGCTGGCTCGATTACAACACCACCTCCCCTAGATGAGACGATGCAGATCT >oligo3 AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCAGGTCTCAGGATCCCCT >oligo4 ACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTT >oligo5 ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAG >oligo6 ATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCA >oligo7 CATCTTTACGGCAGAAGATAATTCTGATGTAGCTCCCAAATGGTCTGTTACATT >oligo8 GAAGCGTTTCTATTACCAAAGAACTCTACATTATATCTTTGATTTGGAGTACCCT >oligo9 GGCTGTTACAGATGGCATTGAAACTTTTGGTGCCCAGTTGGCTTTTGCCAATCC >oligo10 GTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCA >oligo11 TAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGG >oligo12 TCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATG >oligo13 TGACTTCAACGGTCAATTGTTTCTTTGAAACTGTCAATTTAGGAGCTTGAATACC >oligo14 TTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACC >oligo15 TTGATTAGGAATAGCATTGCAATTTTGTTGATTTGGTTGAGTACAGGTTTTCTGT >oligo16 TTGGTGATCGGTTACTACGACTATAGAACCTAAGTATTGTTCTGCTTCTGAAGAT >quiC4-NewForward ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATC >quiC4-NewReverse gactgAGATCTCGTCTCAGGTCTCAGGATCCCCTCATC pca1: ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCATGGATCCTGGGCACAGGAAAGATACTT pca2: ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGAGATCTcagtc final product: GATCTtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacGGATCTGAAAGAGGAGAAAGGATCTatggcgagtagcgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaGGATCTccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataGGATCCtaaCTCGAGctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGA
