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| <pre> | | [[SBB13Ntbk - Mar7 | March 7, 2013]] <br> |
| PCA quiC-4 - oligo1-16 (464bp, pca1)
| | [[SBB13Ntbk - Mar8 | March 8, 2013]] <br> |
| PCR pca1 with quiC-NewForward/Reverse (418bp, pca2)
| | [[SBB13Ntbk - Mar12 | March 12, 2013]] <br> |
| Digest pca2 (EcoRI/BglII, 402+10+6,L, pcrdig)
| | [[SBB13Ntbk - Mar14 | March 14, 2013]] <br> |
| Digest pBca9145-Bca144 (EcoRI/BglII, 2958+9, L, vectdig)
| | [[SSB13Ntbk - Mar19 | March 19, 2013]] <br> |
| Ligate pcrdig and vectdig (pBca9145-quiC4)
| | [[SSB13Ntbk - Mar21 | March 21, 2013]] <br> |
| ------------------------- | | [[SSB13Ntbk - Apr2 | April 2, 2013]] <br> |
| >oligo1
| | [[SSB13Ntbk - Apr16 | April 16, 2013]] <br> |
| AAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTAC
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| >oligo2
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| AACTTTGCTGGCTCGATTACAACACCACCTCCCCTAGATGAGACGATGCAGATCT
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| >oligo3
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| AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCAGGTCTCAGGATCCCCT
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| >oligo4
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| ACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTT
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| >oligo5
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| ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAG
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| >oligo6
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| ATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCA
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| >oligo7
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| CATCTTTACGGCAGAAGATAATTCTGATGTAGCTCCCAAATGGTCTGTTACATT
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| >oligo8
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| GAAGCGTTTCTATTACCAAAGAACTCTACATTATATCTTTGATTTGGAGTACCCT
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| >oligo9
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| GGCTGTTACAGATGGCATTGAAACTTTTGGTGCCCAGTTGGCTTTTGCCAATCC
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| >oligo10
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| GTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCA
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| >oligo11
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| TAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGG
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| >oligo12
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| TCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATG
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| >oligo13
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| TGACTTCAACGGTCAATTGTTTCTTTGAAACTGTCAATTTAGGAGCTTGAATACC
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| >oligo14
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| TTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACC
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| >oligo15
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| TTGATTAGGAATAGCATTGCAATTTTGTTGATTTGGTTGAGTACAGGTTTTCTGT
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| >oligo16 | |
| TTGGTGATCGGTTACTACGACTATAGAACCTAAGTATTGTTCTGCTTCTGAAGAT
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| >quiC4-NewForward
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| ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATC
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| >quiC4-NewReverse
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| gactgAGATCTCGTCTCAGGTCTCAGGATCCCCTCATC
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| pca1:
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| ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCATGGATCCTGGGCACAGGAAAGATACTT
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| pca2: ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGAGATCTcagtc
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| final product:
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| GATCTtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacGGATCTGAAAGAGGAGAAAGGATCTatggcgagtagcgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaGGATCTccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataGGATCCtaaCTCGAGctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGA
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| </pre> | |
