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| [[SBB13Ntbk - Mar7 | March 7, 2013]] <br> | | [[SBB13Ntbk - Mar7 | March 7, 2013]] <br> |
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| <pre>
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| PCA quiC-4 - oligo1-16 (464bp, pca1)
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| PCR pca1 with quiC-NewForward/Reverse (418bp, pca2)
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| Digest pca2 (EcoRI/BglII, 402+10+6,L, pcrdig)
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| Digest pBca9145-Bca144 (EcoRI/BglII, 2958+9, L, vectdig)
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| Ligate pcrdig and vectdig (pBca9145-quiC4)
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| -------------------------
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| >oligo1
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| AAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTAC
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| >oligo2
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| AACTTTGCTGGCTCGATTACAACACCACCTCCCCTAGATGAGACGATGCAGATCT
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| >oligo3
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| AAGTATCTTTCCTGTGCCCAGGATCCATGCCGTCTCAGGTCTCAGGATCCCCT
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| >oligo4
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| ACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTT
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| >oligo5
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| ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAG
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| >oligo6
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| ATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCA
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| >oligo7
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| CATCTTTACGGCAGAAGATAATTCTGATGTAGCTCCCAAATGGTCTGTTACATT
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| >oligo8
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| GAAGCGTTTCTATTACCAAAGAACTCTACATTATATCTTTGATTTGGAGTACCCT
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| >oligo9
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| GGCTGTTACAGATGGCATTGAAACTTTTGGTGCCCAGTTGGCTTTTGCCAATCC
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| >oligo10
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| GTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCA
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| >oligo11
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| TAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGG
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| >oligo12
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| TCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATG
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| >oligo13
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| TGACTTCAACGGTCAATTGTTTCTTTGAAACTGTCAATTTAGGAGCTTGAATACC
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| >oligo14
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| TTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACC
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| >oligo15
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| TTGATTAGGAATAGCATTGCAATTTTGTTGATTTGGTTGAGTACAGGTTTTCTGT
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| >oligo16
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| TTGGTGATCGGTTACTACGACTATAGAACCTAAGTATTGTTCTGCTTCTGAAGAT
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| >quiC4-NewForward
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| ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATC
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| >quiC4-NewReverse
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| gactgAGATCTCGTCTCAGGTCTCAGGATCCCCTCATC
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| pca1:
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| ATATAGATGCCGTCCTAGCGAATTCATGAGATCTGCATCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGGCATGGATCCTGGGCACAGGAAAGATACTT
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| pca2: ccaaaGAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGAGATCTcagtc
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| final product:
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| GATCTtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacGGATCTGAAAGAGGAGAAAGGATCTatggcgagtagcgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaGGATCTccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataGGATCCtaaCTCGAGctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggAATTCCGTCTCATCTAGGGGAGGTGGTGTTGTAATCGAGCCAGCAAAGTTACAGAAAACCTGTACTCAACCAAATCAACAAAATTGCAATGCTATTCCTAATCAAGGTATTCAAGCTCCTAAATTGACAGTTTCAAAGAAACAATTGACCGTTGAAGTCAAGGGTACTCCAAATCAAAGATATAATGTAGAGTTCTTTGGTAATAGAAACGCTTCATCTTCAGAAGCAGAACAATACTTAGGTTCTATAGTCGTAGTAACCGATCACCAAGGATTGGCAAAAGCCAACTGGGCACCAAAAGTTTCAATGCCATCTGTAACAGCCAATGTAACAGACCATTTGGGAGCTACATCAGAATTATCTTCTGCCGTAAAGATGAGGGGATCCTGAGACCTGAGACGA
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| </pre>
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