SBB12 gels

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SynBioBootcamp Homepage
How to Load, Run, and Upload Gels

Ladder

Unless otherwise noted, we will be using the NEB 2-log ladder. NEB site
Image:NEB_2-log_ladder.gif

Example

Image:Invitrogen1kbplus.jpg

JCAnderson 14:40, 17 February 2011 (EST)

021711-AnalGel1 (just an example)

This one used 1kb plus.



Gels: Feb 17

MW ladder used in these gels

Image:MW-ladder.jpg

Au Duong 13:35, 17 February 2012 (EST)

ClassGel1 2_17_2012
Image:gel1 from class 2_17_2012.jpg

Zachary Russ 13:43, 17 February 2012 (EST)

ClassGel2 Image:gel2 from class 2_17_2012.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
JW AJW BMMemptyMW ladderJSDC ADC Bemptyempty


Gels: Feb 21

Tina Nie 13:56, 21 February 2012 (EST)

Class Gel 1
Image:2012_02_21_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Au PCA2TN sbb1213DC ADC B2-log ladderJSMMJWRO TPRO CT

Tina Nie 14:10, 21 February 2012 (EST)

ClassGel2
Image:2012_02_21_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
LZ AAAA PCA2sbb1224sbb12032-log ladderAJ pca2MDJK caff-PCA2AJ AAJ BPR PCR1


Gels: Feb 23

Class Gel #1
Image:NEB_2-log_ladder.gifImage:2012_02_23_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
MMPR-PCAJSTar dig2-log ladderJkotker LZ (1204)MD PhoAsbb1224MD LZMH PCR A+B

Class Gel #2
Image:NEB_2-log_ladder.gifImage:2012_02_23_gel2_ssb2012spring_lo.jpgImage:2012_02_23_gel2_ssb2012spring_hi.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
GF 438GF 2801emptyJKotker Caff (1223)empty2-log ladderPR-PCRKaren PCRproduct empty Au SOE digest

Gels: Feb 24

Gel 1

Image:NEB_2-log_ladder.gifImage:2012_02_24_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
MH PCRPDT 1218MH PCRDIG 1218emptyDC SB 1221LadderemptyemptyemptyGF PCARO SOE3 1231

Gels: Feb 28

Au Duong 14:19, 28 February 2012 (EST)

ClassGel1 2_28_2012
Image:NEB_2-log_ladder.gifImage:gel1 from class 2_28_2012.jpg
\

Robert D O'Dowd 14:19, 28 February 2012 (EST)

Class Gel #2
Image:NEB_2-log_ladder.gifImage:2012_02_28_gel2_ssb2012spring.jpgImage:2012_02_28_gel2_ssb2012spring_lo.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
emptyAJ A+B pcrpdtemptyRD 1231 digempty2-log ladderemptyemptyemptyempty


Gels: Mar 1

Class Gel #1

Image:NEB_2-log_ladder.gifImage:2012_03_1_gel1_ssb2012spring.jpg


Class Gel #2

Image:NEB_2-log_ladder.gifImage:2012_03_01_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Karin 12061224(1)1224(2)1224(3)1203(1)ladderAJRO TM PhoIRO CITMBad Load


Gels:March 1 (Gel 3)

Image:NEB_2-log_ladder.gifImage:2012_03_01_gel3_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
entry onetwo1217fourladderLz-AAAA1(1)Lz-AAAA1(2eightnineten


Gels: Mar 2

Gel 1

Image:NEB_2-log_ladder.gifImage:2012_03_02_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Au SOE3AJ A+BemptyDC A+B2logemptyemptyemptyJW A22log

Gel 2

Image:NEB_2-log_ladder.gifImage:2012_03_02_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+BAJ A+BMM 1232-1MM 1232-2MM 1215-2RO MP 1214LadderMDP2MDP1MDL2

Gel 3

Image:NEB_2-log_ladder.gifImage:2012_03_02_gel3_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
KA miniprep1 1206KA miniprep2 1206KA miniprep3 1206HH Tar 12log ladderHH Tar 2HH LZ 1HH LZ 2MH 1207-1MH 1207-2


Gels: Mar 6

Gel 1

Image:NEB_2-log_ladder.gifImage:2012_03_05_gel1_ssb2012spring.jpg

Class Gel 2

Image:NEB_2-log_ladder.gifImage:2012_03_06_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+BJS 1201-1JS 1201-2JS 1226-1JS 1226-2LadderPR 02-1PR 02-2PR 30-1JK 1233-1


Gel 3

Image:NEB_2-log_ladder.gifImage:2012_03_05_gel3_ssb2012spring.jpg

Class Gel 4

Image:NEB_2-log_ladder.gifImage:2012_03_06_gel4_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
MM 1232-1MM 1232-2MM 1215-2DC 1221 A+ B PurLadderJK 1233-2JK 1204-1JK 1204-2PR 30-2PR 30-3

Class Gel 5

Image:NEB_2-log_ladder.gifImage:2012_03_06_gel5_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
PR 30-4PR 02-1PR 02-2PR 02-3PR 02-4LadderJK 1223-1AJ 12205-1AJ 12205-2AJ 12205-3

Gels: Mar 8

Class Gel 1

Image:NEB_2-log_ladder.gifImage:2012_03_08_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Ladder 2μLGF PCATest Ladder 1:5 10μLJW A4Ladder 3μLLZ AAAA-1JW A41224 M11224 M2MH A+B pcrpdt

Class Gel 2

Image:NEB_2-log_ladder.gifImage:2012_03_08_gel2_ssb2012spring.jpg

Class Gel 3

Image:NEB_2-log_ladder.gifImage:2012_03_08_gel3_ssb2012spring.jpgImage:2012_03_08_gel3_ssb2012spring_hi.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
AJ Digest 1AJ Digest 2AJ Digest 3AJ Digest 4Test Ladder 10μLTN sbb1227 3TN sbb1227 4JK 1204-1AJ A+B Digest (cut)Test Ladder 10μL



Gels: Mar 13

Class Gel 1

Image:NEB_2-log_ladder.gifImage:2012_03_13_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
Karen A sbb1217 mp1Karen A sbb1217 mp2Karen A sbb1217 mp3Karen A sbb1217 mp4Ladersbb1224(1)sbb1224(2)AJ A+BKaren A sbb1217 mp3Ladder

Class Gel 2

Image:NEB_2-log_ladder.gifImage:2012_03_13_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
DC sbb1221 A+DMSODC sbb1221 ADC sbb1221 B+DMSODC sbb1221 BLaderMH 1218 digJV A5emptyemptyempty


Class Gel 3

Image:NEB_2-log_ladder.gifImage:2012_03_13_gel3_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
RO 1214 DigRO 1231 DigAJ A+B PCR digest****LaderKAKAKAKAladder


Gels: Mar 15

Class Gel 1

Image:NEB_2-log_ladder.gifImage:2012_03_15_gel1_ssb2012spring.jpg

Class Gel #2

Image:NEB_2-log_ladder.gifImage:2012_03_15_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1203(1)sbb1203(2)sbb1203(3)sbb1203(4)laddersbb1224(1)sbb1224(1sbb1224(2)sbb1224(3)sbb1224(4)


Karen D. Alvarez 15:34, 15 March 2012 (EDT)

Class Gel #3

Image:NEB_2-log_ladder.gifImage:2012_03_15_gel3_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1217(1)sbb1217(2)sbb1217(3)sbb1217(4)ladderladder -- -- -- --

Gels: March 20

Class Gel 1

Image:NEB_2-log_ladder.gifImage:2012_03_20_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
noneMM 1MM 2MM 3MM 4ladderDC A+BAJ gel purifyGF pcrpdtnone

Class Gel 2

Image:NEB_2-log_ladder.gifImage:2012_03_20_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
LadderemptyJW BDemptyLadderemptyJW A6-BLadderemptyJW A6-B

Class Gel 3

Image:NEB_2-log_ladder.gifImage:2012_03_20_gel3_ssb2012spring labeled.jpg


Gels: March 22

Class Gel 1

Image:NEB_2-log_ladder.gifImage:2012_03_22_gel1_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
noneRO 1214 1RO 1214 2RO 1214 3RO 1214 42-logAJ digestDC A+BJW A10none

Class Gel 2

Image:NEB_2-log_ladder.gifImage:2012_03_22_gel2_ssb2012spring.jpg

Lanes:

1 2 3 4 5 6 7 8 9 10
nonenonenonenone2-logDC A+B Digestnonenonenonenone
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