SBB12 gels: Difference between revisions

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!width="1"|10
!width="1"|10
|-
|-
||1||2||3||4||5||6||7||8||9||10
||MH PCRPDT 1218||MH PCRDIG 1218||empty||DC SB 1221||Ladder||empty||empty||empty||GF PCA||RO SOE3 1231
|}
|}
==Gels: Feb 28==
==Gels: Feb 28==
Line 150: Line 150:
ClassGel1 2_28_2012 <br>
ClassGel1 2_28_2012 <br>
[[Image:NEB_2-log_ladder.gif]][[Image:gel1 from class 2_28_2012.jpg]]<br>\
[[Image:NEB_2-log_ladder.gif]][[Image:gel1 from class 2_28_2012.jpg]]<br>\
Lanes:<br>
 
{| border="1" cellpadding="2"
!width="1"|1
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|-
||1||2||3||4||5||6||7||8||9||10
|}
===[[User:Robert D O'Dowd|Robert D O'Dowd]] 14:19, 28 February 2012 (EST)===
===[[User:Robert D O'Dowd|Robert D O'Dowd]] 14:19, 28 February 2012 (EST)===
Class Gel #2<br>
Class Gel #2<br>
Line 189: Line 175:
===Class Gel #1===
===Class Gel #1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_1_gel1_ssb2012spring.jpg]]<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_1_gel1_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
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!width="1"|8
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!width="1"|10
|-
||1||2||3||4||5||6||7||8||9||10
|}




Line 396: Line 366:


===Class Gel 3===
===Class Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_08_gel3_ssb2012spring.jpg]]<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_08_gel3_ssb2012spring.jpg]][[Image:2012_03_08_gel3_ssb2012spring_hi.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 412: Line 382:
|-
|-
||AJ Digest 1||AJ Digest 2||AJ Digest 3||AJ Digest 4||Test Ladder 10μL||TN sbb1227 3||TN sbb1227 4||JK 1204-1||AJ A+B Digest (cut)||Test Ladder 10μL
||AJ Digest 1||AJ Digest 2||AJ Digest 3||AJ Digest 4||Test Ladder 10μL||TN sbb1227 3||TN sbb1227 4||JK 1204-1||AJ A+B Digest (cut)||Test Ladder 10μL
|}
==Gels: Mar 13==
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_13_gel1_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||Karen A sbb1217 mp1||Karen A sbb1217 mp2||Karen A sbb1217 mp3||Karen A sbb1217 mp4||Lader||sbb1224(1)||sbb1224(2)||AJ A+B||Karen A sbb1217 mp3||Ladder
|}
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_13_gel2_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||DC sbb1221 A+DMSO||DC sbb1221 A||DC sbb1221 B+DMSO||DC sbb1221 B||Lader||MH 1218 dig||JV A5||empty||empty||empty
|}
===Class Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_13_gel3_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||RO 1214 Dig||RO 1231 Dig||AJ A+B PCR digest||****||Lader||KA||KA||KA||KA||ladder
|}
==Gels: Mar 15==
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_15_gel1_ssb2012spring.jpg]]<br>
===Class Gel #2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_15_gel2_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||sbb1203(1)||sbb1203(2)||sbb1203(3)||sbb1203(4)||ladder||sbb1224(1)||sbb1224(1||sbb1224(2)||sbb1224(3)||sbb1224(4)
|}
[[User:Karen D. Alvarez|Karen D. Alvarez]] 15:34, 15 March 2012 (EDT)
===Class Gel #3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_15_gel3_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||sbb1217(1)||sbb1217(2)||sbb1217(3)||sbb1217(4)||ladder||ladder|| -- || -- || -- || --
|}
==Gels: March 20==
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_20_gel1_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||none||MM 1||MM 2||MM 3||MM 4||ladder||DC A+B||AJ gel purify||GF pcrpdt||none
|}
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_20_gel2_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||Ladder||empty||JW BD||empty||Ladder||empty||JW A6-B||Ladder||empty||JW A6-B
|}
===Class Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_20_gel3_ssb2012spring labeled.jpg]]<br>
==Gels: March 22==
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_22_gel1_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||none||RO 1214 1||RO 1214 2||RO 1214 3||RO 1214 4||2-log||AJ digest||DC A+B||JW A10||none
|}
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_22_gel2_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||none||none||none||none||2-log||DC A+B Digest||none||none||none||none
|}
|}

Latest revision as of 10:37, 25 March 2012

SynBioBootcamp Homepage
How to Load, Run, and Upload Gels

Ladder

Unless otherwise noted, we will be using the NEB 2-log ladder. NEB site

Example


JCAnderson 14:40, 17 February 2011 (EST)

021711-AnalGel1 (just an example)

This one used 1kb plus.



Gels: Feb 17

MW ladder used in these gels

Au Duong 13:35, 17 February 2012 (EST)

ClassGel1 2_17_2012

Zachary Russ 13:43, 17 February 2012 (EST)

ClassGel2

Lanes:

1 2 3 4 5 6 7 8 9 10
JW A JW B MM empty MW ladder JS DC A DC B empty empty


Gels: Feb 21

Tina Nie 13:56, 21 February 2012 (EST)

Class Gel 1

Lanes:

1 2 3 4 5 6 7 8 9 10
Au PCA2 TN sbb1213 DC A DC B 2-log ladder JS MM JW RO TP RO CT

Tina Nie 14:10, 21 February 2012 (EST)

ClassGel2

Lanes:

1 2 3 4 5 6 7 8 9 10
LZ AAAA PCA2 sbb1224 sbb1203 2-log ladder AJ pca2 MD JK caff-PCA2 AJ A AJ B PR PCR1


Gels: Feb 23

Class Gel #1

Lanes:

1 2 3 4 5 6 7 8 9 10
MM PR-PCA JS Tar dig 2-log ladder Jkotker LZ (1204) MD PhoA sbb1224 MD LZ MH PCR A+B

Class Gel #2

Lanes:

1 2 3 4 5 6 7 8 9 10
GF 438 GF 2801 empty JKotker Caff (1223) empty 2-log ladder PR-PCR Karen PCRproduct empty Au SOE digest

Gels: Feb 24

Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
MH PCRPDT 1218 MH PCRDIG 1218 empty DC SB 1221 Ladder empty empty empty GF PCA RO SOE3 1231

Gels: Feb 28

Au Duong 14:19, 28 February 2012 (EST)

ClassGel1 2_28_2012

\

Robert D O'Dowd 14:19, 28 February 2012 (EST)

Class Gel #2

Lanes:

1 2 3 4 5 6 7 8 9 10
empty AJ A+B pcrpdt empty RD 1231 dig empty 2-log ladder empty empty empty empty


Gels: Mar 1

Class Gel #1



Class Gel #2


Lanes:

1 2 3 4 5 6 7 8 9 10
Karin 1206 1224(1) 1224(2) 1224(3) 1203(1) ladder AJ RO TM PhoI RO CITM Bad Load


Gels:March 1 (Gel 3)


Lanes:

1 2 3 4 5 6 7 8 9 10
entry one two 1217 four ladder Lz-AAAA1(1) Lz-AAAA1(2 eight nine ten


Gels: Mar 2

Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
Au SOE3 AJ A+B empty DC A+B 2log empty empty empty JW A2 2log

Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+B AJ A+B MM 1232-1 MM 1232-2 MM 1215-2 RO MP 1214 Ladder MDP2 MDP1 MDL2

Gel 3


Lanes:

1 2 3 4 5 6 7 8 9 10
KA miniprep1 1206 KA miniprep2 1206 KA miniprep3 1206 HH Tar 1 2log ladder HH Tar 2 HH LZ 1 HH LZ 2 MH 1207-1 MH 1207-2


Gels: Mar 6

Gel 1


Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+B JS 1201-1 JS 1201-2 JS 1226-1 JS 1226-2 Ladder PR 02-1 PR 02-2 PR 30-1 JK 1233-1


Gel 3


Class Gel 4


Lanes:

1 2 3 4 5 6 7 8 9 10
MM 1232-1 MM 1232-2 MM 1215-2 DC 1221 A+ B Pur Ladder JK 1233-2 JK 1204-1 JK 1204-2 PR 30-2 PR 30-3

Class Gel 5


Lanes:

1 2 3 4 5 6 7 8 9 10
PR 30-4 PR 02-1 PR 02-2 PR 02-3 PR 02-4 Ladder JK 1223-1 AJ 12205-1 AJ 12205-2 AJ 12205-3

Gels: Mar 8

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
Ladder 2μL GF PCA Test Ladder 1:5 10μL JW A4 Ladder 3μL LZ AAAA-1 JW A4 1224 M1 1224 M2 MH A+B pcrpdt

Class Gel 2


Class Gel 3


Lanes:

1 2 3 4 5 6 7 8 9 10
AJ Digest 1 AJ Digest 2 AJ Digest 3 AJ Digest 4 Test Ladder 10μL TN sbb1227 3 TN sbb1227 4 JK 1204-1 AJ A+B Digest (cut) Test Ladder 10μL



Gels: Mar 13

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
Karen A sbb1217 mp1 Karen A sbb1217 mp2 Karen A sbb1217 mp3 Karen A sbb1217 mp4 Lader sbb1224(1) sbb1224(2) AJ A+B Karen A sbb1217 mp3 Ladder

Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
DC sbb1221 A+DMSO DC sbb1221 A DC sbb1221 B+DMSO DC sbb1221 B Lader MH 1218 dig JV A5 empty empty empty


Class Gel 3


Lanes:

1 2 3 4 5 6 7 8 9 10
RO 1214 Dig RO 1231 Dig AJ A+B PCR digest **** Lader KA KA KA KA ladder


Gels: Mar 15

Class Gel 1


Class Gel #2


Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1203(1) sbb1203(2) sbb1203(3) sbb1203(4) ladder sbb1224(1) sbb1224(1 sbb1224(2) sbb1224(3) sbb1224(4)


Karen D. Alvarez 15:34, 15 March 2012 (EDT)

Class Gel #3


Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1217(1) sbb1217(2) sbb1217(3) sbb1217(4) ladder ladder -- -- -- --

Gels: March 20

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
none MM 1 MM 2 MM 3 MM 4 ladder DC A+B AJ gel purify GF pcrpdt none

Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
Ladder empty JW BD empty Ladder empty JW A6-B Ladder empty JW A6-B

Class Gel 3



Gels: March 22

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
none RO 1214 1 RO 1214 2 RO 1214 3 RO 1214 4 2-log AJ digest DC A+B JW A10 none

Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
none none none none 2-log DC A+B Digest none none none none
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