SBB12 gels: Difference between revisions

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Line 104: Line 104:
!width="1"|10
!width="1"|10
|-
|-
||MM||PR||JS||Tar dig||2-log ladder||Jkotker LZ (1204)||MD PhoA||sbb1224||MD LZ||MH PCR A+B
||MM||PR-PCA||JS||Tar dig||2-log ladder||Jkotker LZ (1204)||MD PhoA||sbb1224||MD LZ||MH PCR A+B
|}
|}


Class Gel #1<br>
Class Gel #2<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_02_23_gel2_ssb2012spring_lo.jpg]][[Image:2012_02_23_gel2_ssb2012spring_hi.jpg]]<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_02_23_gel2_ssb2012spring_lo.jpg]][[Image:2012_02_23_gel2_ssb2012spring_hi.jpg]]<br>


Line 123: Line 123:
!width="1"|10
!width="1"|10
|-
|-
||GF 438||GF 2801||empty||JKotker Caff (1223)||empty||2-log ladder||PR||Karen PCRproduct|| empty ||Au SOE digest
||GF 438||GF 2801||empty||JKotker Caff (1223)||empty||2-log ladder||PR-PCR||Karen PCRproduct|| empty ||Au SOE digest
|}
|}
==Gels: Feb 24==
==Gels: Feb 24==
===Gel 1===
===Gel 1===
Line 142: Line 143:
!width="1"|10
!width="1"|10
|-
|-
||1||2||3||4||5||6||7||8||9||10
||MH PCRPDT 1218||MH PCRDIG 1218||empty||DC SB 1221||Ladder||empty||empty||empty||GF PCA||RO SOE3 1231
|}
|}
==Gels: Feb 28==
==Gels: Feb 28==
Line 149: Line 150:
ClassGel1 2_28_2012 <br>
ClassGel1 2_28_2012 <br>
[[Image:NEB_2-log_ladder.gif]][[Image:gel1 from class 2_28_2012.jpg]]<br>\
[[Image:NEB_2-log_ladder.gif]][[Image:gel1 from class 2_28_2012.jpg]]<br>\
===[[User:Robert D O'Dowd|Robert D O'Dowd]] 14:19, 28 February 2012 (EST)===
Class Gel #2<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_02_28_gel2_ssb2012spring.jpg]][[Image:2012_02_28_gel2_ssb2012spring_lo.jpg]]<br>
Lanes:<br>
Lanes:<br>
{| border="1" cellpadding="2"
{| border="1" cellpadding="2"
Line 162: Line 168:
!width="1"|10
!width="1"|10
|-
|-
||1||2||3||4||5||6||7||8||9||10
||empty||AJ A+B pcrpdt||empty||RD 1231 dig||empty||2-log ladder||empty||empty||empty||empty
|}
|}
===[[User:Robert D O'Dowd|Robert D O'Dowd]] 14:19, 28 February 2012 (EST)===
 
Class Gel #2<br>
 
[[Image:NEB_2-log_ladder.gif]][[Image:2012_02_28_gel2_ssb2012spring.jpg]][[Image:2012_02_28_gel2_ssb2012spring_lo.jpg]]<br>
==Gels: Mar 1==
===Class Gel #1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_1_gel1_ssb2012spring.jpg]]<br>
 
 
===Class Gel #2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_01_gel2_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||Karin 1206||1224(1)||1224(2)||1224(3)||1203(1)||ladder||AJ||RO TM PhoI||RO CITM||Bad Load
|}
 
 
== Gels:March 1 (Gel 3)<br>==
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_01_gel3_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||entry one||two||1217||four||ladder||Lz-AAAA1(1)||Lz-AAAA1(2||eight||nine||ten
|}
 
 
 
==Gels: Mar 2==
===Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_02_gel1_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||Au SOE3||AJ A+B||empty||DC A+B||2log||empty||empty||empty||JW A2||2log
|}
 
===Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_02_gel2_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||AJ A+B||AJ A+B||MM 1232-1||MM 1232-2||MM 1215-2||RO MP 1214||Ladder||MDP2||MDP1||MDL2
|}
 
===Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_02_gel3_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||KA miniprep1 1206||KA miniprep2 1206||KA miniprep3 1206||HH Tar 1||2log ladder||HH Tar 2||HH LZ 1||HH LZ 2||MH 1207-1||MH 1207-2
|}
 
 
==Gels: Mar 6==
===Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_05_gel1_ssb2012spring.jpg]]<br>
 
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel2_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||AJ A+B||JS 1201-1||JS 1201-2||JS 1226-1||JS 1226-2||Ladder||PR 02-1||PR 02-2||PR 30-1||JK 1233-1
|}
 
 
===Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_05_gel3_ssb2012spring.jpg]]<br>
 
===Class Gel 4===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel4_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||MM 1232-1||MM 1232-2||MM 1215-2||DC 1221 A+ B Pur||Ladder||JK 1233-2||JK 1204-1||JK 1204-2||PR 30-2||PR 30-3
|}
 
===Class Gel 5===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_06_gel5_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||PR 30-4||PR 02-1||PR 02-2||PR 02-3||PR 02-4||Ladder||JK 1223-1||AJ 12205-1||AJ 12205-2||AJ 12205-3
|}
==Gels: Mar 8==
 
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_08_gel1_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||Ladder 2μL||GF PCA||Test Ladder 1:5 10μL||JW A4||Ladder 3μL||LZ AAAA-1||JW A4||1224 M1||1224 M2||MH A+B pcrpdt
|}
 
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_08_gel2_ssb2012spring.jpg]]<br>
 
===Class Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_08_gel3_ssb2012spring.jpg]][[Image:2012_03_08_gel3_ssb2012spring_hi.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 181: Line 381:
!width="1"|10
!width="1"|10
|-
|-
||empty||AJ A+B pcrpdt||empty||RD 1231 dig||empty||2-log ladder||empty||empty||empty||empty
||AJ Digest 1||AJ Digest 2||AJ Digest 3||AJ Digest 4||Test Ladder 10μL||TN sbb1227 3||TN sbb1227 4||JK 1204-1||AJ A+B Digest (cut)||Test Ladder 10μL
|}
|}




==Gels: Mar 1==
 
===Class Gel #1===
 
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_1_gel1_ssb2012spring.jpg]]<br>
==Gels: Mar 13==
 
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_13_gel1_ssb2012spring.jpg]]<br>
 
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||Karen A sbb1217 mp1||Karen A sbb1217 mp2||Karen A sbb1217 mp3||Karen A sbb1217 mp4||Lader||sbb1224(1)||sbb1224(2)||AJ A+B||Karen A sbb1217 mp3||Ladder
|}
 
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_13_gel2_ssb2012spring.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 202: Line 424:
!width="1"|10
!width="1"|10
|-
|-
||1||2||3||4||5||6||7||8||9||10
||DC sbb1221 A+DMSO||DC sbb1221 A||DC sbb1221 B+DMSO||DC sbb1221 B||Lader||MH 1218 dig||JV A5||empty||empty||empty
|}
|}




===Class Gel #2===
===Class Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_01_gel2_ssb2012spring.jpg]]<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_13_gel3_ssb2012spring.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 222: Line 444:
!width="1"|10
!width="1"|10
|-
|-
||Karin 1206||1224(1)||1224(2)||1224(3)||1203(1)||ladder||AJ||RO TM PhoI||RO CITM||Bad Load
||RO 1214 Dig||RO 1231 Dig||AJ A+B PCR digest||****||Lader||KA||KA||KA||KA||ladder
|}
|}




== Gels:March 1 (Gel 3)<br>==
 
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_01_gel3_ssb2012spring.jpg]]<br>
==Gels: Mar 15==
 
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_15_gel1_ssb2012spring.jpg]]<br>
 
===Class Gel #2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_15_gel2_ssb2012spring.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 233: Line 461:
!width="1"|1
!width="1"|1
!width="1"|2
!width="1"|2
!width="1"|3  
!width="1"|3
!width="1"|4
!width="1"|4
!width="1"|5
!width="1"|5
!width="1"|6  
!width="1"|6
!width="1"|7  
!width="1"|7
!width="1"|8
!width="1"|8
!width="1"|9
!width="1"|9
!width="1"|10
!width="1"|10
|-
|-
||entry one||two||1217||four||ladder||Lz-AAAA1(1)||Lz-AAAA1(2||eight||nine||ten
||sbb1203(1)||sbb1203(2)||sbb1203(3)||sbb1203(4)||ladder||sbb1224(1)||sbb1224(1||sbb1224(2)||sbb1224(3)||sbb1224(4)
|}
|}




[[User:Karen D. Alvarez|Karen D. Alvarez]] 15:34, 15 March 2012 (EDT)


==Gels: Mar 2==
===Class Gel #3===
===Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_15_gel3_ssb2012spring.jpg]]<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_02_gel1_ssb2012spring.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 264: Line 492:
!width="1"|10
!width="1"|10
|-
|-
||Au SOE3||AJ A+B||empty||DC A+B||2log||empty||empty||empty||JW A2||2log
||sbb1217(1)||sbb1217(2)||sbb1217(3)||sbb1217(4)||ladder||ladder|| -- || -- || -- || --
|}
|}


===Gel 2===
==Gels: March 20==
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_02_gel2_ssb2012spring.jpg]]<br>
 
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_20_gel1_ssb2012spring.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 283: Line 513:
!width="1"|10
!width="1"|10
|-
|-
||AJ A+B||AJ A+B||MM 1232-1||MM 1232-2||MM 1215-2||RO MP 1214||Ladder||MDP2||MDP1||MDL2
||none||MM 1||MM 2||MM 3||MM 4||ladder||DC A+B||AJ gel purify||GF pcrpdt||none
|}
|}


===Gel 3===
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_02_gel3_ssb2012spring.jpg]]<br>
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_20_gel2_ssb2012spring.jpg]]<br>


Lanes:<br>
Lanes:<br>
Line 302: Line 532:
!width="1"|10
!width="1"|10
|-
|-
||KA miniprep1 1206||KA miniprep2 1206||KA miniprep3 1206||HH Tar 1||2log ladder||HH Tar 2||HH LZ 1||HH LZ 2||MH 1207-1||MH 1207-2
||Ladder||empty||JW BD||empty||Ladder||empty||JW A6-B||Ladder||empty||JW A6-B
|}
|}


===Class Gel 3===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_20_gel3_ssb2012spring labeled.jpg]]<br>
==Gels: March 22==
===Class Gel 1===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_22_gel1_ssb2012spring.jpg]]<br>
Lanes:<br>
{| border="1" cellpadding="2"
!width="1"|1
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||none||RO 1214 1||RO 1214 2||RO 1214 3||RO 1214 4||2-log||AJ digest||DC A+B||JW A10||none
|}
===Class Gel 2===
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_22_gel2_ssb2012spring.jpg]]<br>


==Gels: Mar 5==
Lanes:<br>
===[[User:Au Duong|Au Duong]] 13:52, 6 March 2012 (EST)===
{| border="1" cellpadding="2"
===Gel 1===
!width="1"|1
[[Image:NEB_2-log_ladder.gif]][[Image:2012_03_05_gel1_ssb2012spring.jpg]]<br>
!width="1"|2
!width="1"|3
!width="1"|4
!width="1"|5
!width="1"|6
!width="1"|7
!width="1"|8
!width="1"|9
!width="1"|10
|-
||none||none||none||none||2-log||DC A+B Digest||none||none||none||none
|}

Latest revision as of 10:37, 25 March 2012

SynBioBootcamp Homepage
How to Load, Run, and Upload Gels

Ladder

Unless otherwise noted, we will be using the NEB 2-log ladder. NEB site

Example


JCAnderson 14:40, 17 February 2011 (EST)

021711-AnalGel1 (just an example)

This one used 1kb plus.



Gels: Feb 17

MW ladder used in these gels

Au Duong 13:35, 17 February 2012 (EST)

ClassGel1 2_17_2012

Zachary Russ 13:43, 17 February 2012 (EST)

ClassGel2

Lanes:

1 2 3 4 5 6 7 8 9 10
JW A JW B MM empty MW ladder JS DC A DC B empty empty


Gels: Feb 21

Tina Nie 13:56, 21 February 2012 (EST)

Class Gel 1

Lanes:

1 2 3 4 5 6 7 8 9 10
Au PCA2 TN sbb1213 DC A DC B 2-log ladder JS MM JW RO TP RO CT

Tina Nie 14:10, 21 February 2012 (EST)

ClassGel2

Lanes:

1 2 3 4 5 6 7 8 9 10
LZ AAAA PCA2 sbb1224 sbb1203 2-log ladder AJ pca2 MD JK caff-PCA2 AJ A AJ B PR PCR1


Gels: Feb 23

Class Gel #1

Lanes:

1 2 3 4 5 6 7 8 9 10
MM PR-PCA JS Tar dig 2-log ladder Jkotker LZ (1204) MD PhoA sbb1224 MD LZ MH PCR A+B

Class Gel #2

Lanes:

1 2 3 4 5 6 7 8 9 10
GF 438 GF 2801 empty JKotker Caff (1223) empty 2-log ladder PR-PCR Karen PCRproduct empty Au SOE digest

Gels: Feb 24

Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
MH PCRPDT 1218 MH PCRDIG 1218 empty DC SB 1221 Ladder empty empty empty GF PCA RO SOE3 1231

Gels: Feb 28

Au Duong 14:19, 28 February 2012 (EST)

ClassGel1 2_28_2012

\

Robert D O'Dowd 14:19, 28 February 2012 (EST)

Class Gel #2

Lanes:

1 2 3 4 5 6 7 8 9 10
empty AJ A+B pcrpdt empty RD 1231 dig empty 2-log ladder empty empty empty empty


Gels: Mar 1

Class Gel #1



Class Gel #2


Lanes:

1 2 3 4 5 6 7 8 9 10
Karin 1206 1224(1) 1224(2) 1224(3) 1203(1) ladder AJ RO TM PhoI RO CITM Bad Load


Gels:March 1 (Gel 3)


Lanes:

1 2 3 4 5 6 7 8 9 10
entry one two 1217 four ladder Lz-AAAA1(1) Lz-AAAA1(2 eight nine ten


Gels: Mar 2

Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
Au SOE3 AJ A+B empty DC A+B 2log empty empty empty JW A2 2log

Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+B AJ A+B MM 1232-1 MM 1232-2 MM 1215-2 RO MP 1214 Ladder MDP2 MDP1 MDL2

Gel 3


Lanes:

1 2 3 4 5 6 7 8 9 10
KA miniprep1 1206 KA miniprep2 1206 KA miniprep3 1206 HH Tar 1 2log ladder HH Tar 2 HH LZ 1 HH LZ 2 MH 1207-1 MH 1207-2


Gels: Mar 6

Gel 1


Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
AJ A+B JS 1201-1 JS 1201-2 JS 1226-1 JS 1226-2 Ladder PR 02-1 PR 02-2 PR 30-1 JK 1233-1


Gel 3


Class Gel 4


Lanes:

1 2 3 4 5 6 7 8 9 10
MM 1232-1 MM 1232-2 MM 1215-2 DC 1221 A+ B Pur Ladder JK 1233-2 JK 1204-1 JK 1204-2 PR 30-2 PR 30-3

Class Gel 5


Lanes:

1 2 3 4 5 6 7 8 9 10
PR 30-4 PR 02-1 PR 02-2 PR 02-3 PR 02-4 Ladder JK 1223-1 AJ 12205-1 AJ 12205-2 AJ 12205-3

Gels: Mar 8

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
Ladder 2μL GF PCA Test Ladder 1:5 10μL JW A4 Ladder 3μL LZ AAAA-1 JW A4 1224 M1 1224 M2 MH A+B pcrpdt

Class Gel 2


Class Gel 3


Lanes:

1 2 3 4 5 6 7 8 9 10
AJ Digest 1 AJ Digest 2 AJ Digest 3 AJ Digest 4 Test Ladder 10μL TN sbb1227 3 TN sbb1227 4 JK 1204-1 AJ A+B Digest (cut) Test Ladder 10μL



Gels: Mar 13

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
Karen A sbb1217 mp1 Karen A sbb1217 mp2 Karen A sbb1217 mp3 Karen A sbb1217 mp4 Lader sbb1224(1) sbb1224(2) AJ A+B Karen A sbb1217 mp3 Ladder

Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
DC sbb1221 A+DMSO DC sbb1221 A DC sbb1221 B+DMSO DC sbb1221 B Lader MH 1218 dig JV A5 empty empty empty


Class Gel 3


Lanes:

1 2 3 4 5 6 7 8 9 10
RO 1214 Dig RO 1231 Dig AJ A+B PCR digest **** Lader KA KA KA KA ladder


Gels: Mar 15

Class Gel 1


Class Gel #2


Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1203(1) sbb1203(2) sbb1203(3) sbb1203(4) ladder sbb1224(1) sbb1224(1 sbb1224(2) sbb1224(3) sbb1224(4)


Karen D. Alvarez 15:34, 15 March 2012 (EDT)

Class Gel #3


Lanes:

1 2 3 4 5 6 7 8 9 10
sbb1217(1) sbb1217(2) sbb1217(3) sbb1217(4) ladder ladder -- -- -- --

Gels: March 20

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
none MM 1 MM 2 MM 3 MM 4 ladder DC A+B AJ gel purify GF pcrpdt none

Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
Ladder empty JW BD empty Ladder empty JW A6-B Ladder empty JW A6-B

Class Gel 3



Gels: March 22

Class Gel 1


Lanes:

1 2 3 4 5 6 7 8 9 10
none RO 1214 1 RO 1214 2 RO 1214 3 RO 1214 4 2-log AJ digest DC A+B JW A10 none

Class Gel 2


Lanes:

1 2 3 4 5 6 7 8 9 10
none none none none 2-log DC A+B Digest none none none none
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