SBB12 gels

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How to Load, Run, and Upload Gels

Ladder

Unless otherwise noted, we will be using the NEB 2-log ladder. NEB site

Example

JCAnderson 14:40, 17 February 2011 (EST)

021711-AnalGel1 (just an example)

This one used 1kb plus.

Gels: Feb 17

MW ladder used in these gels

Au Duong 13:35, 17 February 2012 (EST)

ClassGel1 2_17_2012

Zachary Russ 13:43, 17 February 2012 (EST)

ClassGel2

Lanes:

1	2	3	4	5	6	7	8	9	10
JW A	JW B	MM	empty	MW ladder	JS	DC A	DC B	empty	empty

Gels: Feb 21

Tina Nie 13:56, 21 February 2012 (EST)

Class Gel 1

Lanes:

1	2	3	4	5	6	7	8	9	10
Au PCA2	TN sbb1213	DC A	DC B	2-log ladder	JS	MM	JW	RO TP	RO CT

Tina Nie 14:10, 21 February 2012 (EST)

ClassGel2

Lanes:

1	2	3	4	5	6	7	8	9	10
LZ AAAA PCA2	sbb1224	sbb1203	2-log ladder	AJ pca2	MD	JK caff-PCA2	AJ A	AJ B	PR PCR1

Gels: Feb 23

Class Gel #1

Lanes:

1	2	3	4	5	6	7	8	9	10
MM	PR-PCA	JS	Tar dig	2-log ladder	Jkotker LZ (1204)	MD PhoA	sbb1224	MD LZ	MH PCR A+B

Class Gel #2

Lanes:

1	2	3	4	5	6	7	8	9	10
GF 438	GF 2801	empty	JKotker Caff (1223)	empty	2-log ladder	PR-PCR	Karen PCRproduct	empty	Au SOE digest

Gels: Feb 24

Gel 1

Lanes:

1	2	3	4	5	6	7	8	9	10
1	2	3	4	5	6	7	8	9	10

Gels: Feb 28

Au Duong 14:19, 28 February 2012 (EST)

ClassGel1 2_28_2012

\ Lanes:

1	2	3	4	5	6	7	8	9	10
1	2	3	4	5	6	7	8	9	10

Robert D O'Dowd 14:19, 28 February 2012 (EST)

Class Gel #2

Lanes:

1	2	3	4	5	6	7	8	9	10
empty	AJ A+B pcrpdt	empty	RD 1231 dig	empty	2-log ladder	empty	empty	empty	empty

Gels: Mar 1

Class Gel #1

Lanes:

1	2	3	4	5	6	7	8	9	10
1	2	3	4	5	6	7	8	9	10

Class Gel #2

Lanes:

1	2	3	4	5	6	7	8	9	10
Karin 1206	1224(1)	1224(2)	1224(3)	1203(1)	ladder	AJ	RO TM PhoI	RO CITM	Bad Load

Gels:March 1 (Gel 3)

Lanes:

1	2	3	4	5	6	7	8	9	10
entry one	two	1217	four	ladder	Lz-AAAA1(1)	Lz-AAAA1(2	eight	nine	ten

Gels: Mar 2

Gel 1

Lanes:

1	2	3	4	5	6	7	8	9	10
Au SOE3	AJ A+B	empty	DC A+B	2log	empty	empty	empty	JW A2	2log

Gel 2

Lanes:

1	2	3	4	5	6	7	8	9	10
AJ A+B	AJ A+B	MM 1232-1	MM 1232-2	MM 1215-2	RO MP 1214	Ladder	MDP2	MDP1	MDL2

Gel 3

Lanes:

1	2	3	4	5	6	7	8	9	10
KA miniprep1 1206	KA miniprep2 1206	KA miniprep3 1206	HH Tar 1	2log ladder	HH Tar 2	HH LZ 1	HH LZ 2	MH 1207-1	MH 1207-2

Gels: Mar 6

Gel 1

Class Gel 2

Lanes:

1	2	3	4	5	6	7	8	9	10
AJ A+B	JS 1201-1	JS 1201-2	JS 1226-1	JS 1226-2	Ladder	PR 02-1	PR 02-2	PR 30-1	JK 1233-1

Gel 3

Class Gel 4

Lanes:

1	2	3	4	5	6	7	8	9	10
MM 1232-1	MM 1232-2	MM 1215-2	DC 1221 A+ B Pur	Ladder	JK 1233-2	JK 1204-1	JK 1204-2	PR 30-2	PR 30-3

Class Gel 5

Lanes:

1	2	3	4	5	6	7	8	9	10
PR 30-4	PR 02-1	PR 02-2	PR 02-3	PR 02-4	Ladder	JK 1223-1	AJ 12205-1	AJ 12205-2	AJ 12205-3

Gels: Mar 8

Class Gel 1

Lanes:

1	2	3	4	5	6	7	8	9	10
Ladder 2μL	GF PCA	Test Ladder 1:5 10μL	JW A4	Ladder 3μL	LZ AAAA-1	JW A4	1224 M1	1224 M2	MH A+B pcrpdt

Class Gel 2

Class Gel 3

Lanes:

1	2	3	4	5	6	7	8	9	10
AJ Digest 1	AJ Digest 2	AJ Digest 3	AJ Digest 4	Test Ladder 10μL	TN sbb1227 3	TN sbb1227 4	JK 1204-1	AJ A+B Digest (cut)	Test Ladder 10μL

Gels: Mar 13

Class Gel 1

Lanes:

1	2	3	4	5	6	7	8	9	10
Karen A sbb1217 mp1	Karen A sbb1217 mp2	Karen A sbb1217 mp3	Karen A sbb1217 mp4	Lader	sbb1224(1)	sbb1224(2)	AJ A+B	Karen A sbb1217 mp3	Ladder

Class Gel 2

Lanes:

1	2	3	4	5	6	7	8	9	10
DC sbb1221 A+DMSO	DC sbb1221 A	DC sbb1221 B+DMSO	DC sbb1221 B	Lader	MH 1218 dig	JV A5	empty	empty	empty

Class Gel 3

Lanes:

1	2	3	4	5	6	7	8	9	10
RO 1214 Dig	RO 1231 Dig	AJ A+B PCR digest	****	Lader	KA	KA	KA	KA	ladder

Gels: Mar 15

Class Gel 1

Class Gel #2

Lanes:

1	2	3	4	5	6	7	8	9	10
sbb1203(1)	sbb1203(2)	sbb1203(3)	sbb1203(4)	ladder	sbb1224(1)	sbb1224(1	sbb1224(2)	sbb1224(3)	sbb1224(4)

Karen D. Alvarez 15:34, 15 March 2012 (EDT)

Class Gel #3

Lanes:

1	2	3	4	5	6	7	8	9	10
sbb1217(1)	sbb1217(2)	sbb1217(3)	sbb1217(4)	ladder	ladder	--	--	--	--

Gels: March 20

Class Gel 1

Lanes:

1	2	3	4	5	6	7	8	9	10
none	MM 1	MM 2	MM 3	MM 4	ladder	DC A+B	AJ gel purify	GF pcrpdt	none

Class Gel 2

Lanes:

1	2	3	4	5	6	7	8	9	10
Ladder	empty	JW BD	empty	Ladder	empty	JW A6-B	Ladder	empty	JW A6-B