SBB12Ntbk-Robert D O'Dowd

Robert D O'Dowd 2/16

2/16 sbb1214 PCA on o16, o17, o12. Followed PCA protocol[1]

100uM dilutions of o16 o17 o12.

add nm weight x10= uL of ddH2O.

Label 1214. Place on PCA rack.

sbb1231 PCR on ca998/rdoSOECI_TM-R and pBca 9145-jtk2768 Label CI TM 1231 PCR on rdoSOETM_PhoA-F/g00101 and pBjh 1601CK-Bjh2128 Label TM PhoA 1231

Followed PCR protocol [2]

Place CI TM 1231 on 500-1K bp rack Place Label TM PhoA 1231 on 1K-2K bp rack

Robert D O'Dowd 2/17

2/17 Leucine zipper 1214 Zymo cleanup-small fragment cleanup. Label 1214 PCA1 [3]

Amplification/phusion step. Template DNA added last. [4]

Template material and fragments for SOE placed in Eluded Products box.

Could not conduct SOEPCR gel or setup. Doing next tuesday.