SBB12Ntbk-Robert D O'Dowd: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 27: | Line 27: | ||
2/17 | 2/17 | ||
Leucine zipper 1214 | Leucine zipper 1214 | ||
Zymo cleanup-small fragment cleanup. | |||
Zymo cleanup-small fragment cleanup. [http://openwetware.org/wiki/Template:SBB-Protocols_Zymo2] Label 1214 PCA1 | |||
Amplification/phusion step. Template DNA added last. [http://openwetware.org/wiki/Template:SBB-PCA] | Amplification/phusion step. Template DNA added last. [http://openwetware.org/wiki/Template:SBB-PCA] | ||
Revision as of 03:22, 21 February 2012
~~!~~
Robert D O'Dowd 2/16
2/16 sbb1214 PCA on o16, o17, o12. Followed PCA protocol[1]
100uM dilutions of o16 o17 o12.
add nm weight x10= uL of ddH2O.
Label 1214. Place on PCA rack.
sbb1231
PCR on ca998/rdoSOECI_TM-R and pBca 9145-jtk2768 Label CI TM 1231
PCR on rdoSOETM_PhoA-F/g00101 and pBjh 1601CK-Bjh2128 Label TM PhoA 1231
Followed PCR protocol [2]
Place CI TM 1231 on 500-1K bp rack
Place Label TM PhoA 1231 on 1K-2K bp rack
Robert D O'Dowd 2/17
2/17 Leucine zipper 1214
Zymo cleanup-small fragment cleanup. [3] Label 1214 PCA1
Amplification/phusion step. Template DNA added last. [4]
Template material and fragments for SOE placed in Eluded Products box.
Could not conduct SOEPCR gel or setup. Doing next tuesday.
