SBB12Ntbk-Melvin Du: Difference between revisions
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(New page: February 16, 2012 ----------------- PCR/PCA stuff.) |
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February 16, 2012 | February 16, 2012 | ||
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PCR/PCA | Started working on creating parts as per the design specs and construction files. | ||
Worked first on cloning the PhoA oligos. Followed steps on "Cloning by PCR" page. Performed dilutions: 401uL for PhoA-F, 284 for PhoA-R. Put the final product on ice after mixing. | |||
Proceeded to set up PCA on oligos 01/011/02 for the leucine zipper. Followed the PCA steps on the protocol pages (PCA Gene Synthesis) and let sit on ice. | |||
Revision as of 12:55, 16 February 2012
February 16, 2012
Started working on creating parts as per the design specs and construction files.
Worked first on cloning the PhoA oligos. Followed steps on "Cloning by PCR" page. Performed dilutions: 401uL for PhoA-F, 284 for PhoA-R. Put the final product on ice after mixing.
Proceeded to set up PCA on oligos 01/011/02 for the leucine zipper. Followed the PCA steps on the protocol pages (PCA Gene Synthesis) and let sit on ice.
