SBB12Ntbk-JonWright

Jon Wright 2/16/2012

Diluted JW000R and JW000F to 10uM

Set up PCRs:

24uL ddH2O
3.3uL 10x Expand Buffer "2"
3.3uL dNTPs (2mM in each)
1uL ca998, 10uM
1uL jw000R, 10uM
0.5uL Expand polymerase "1"
0.5uL Plasmid jtk2081

24uL ddH2O
3.3uL 10x Expand Buffer "2"
3.3uL dNTPs (2mM in each)
1uL G00101, 10uM
1uL JW000F, 10uM
0.5uL Expand polymerase "1"
0.5uL Plasmid 3346

Set up PCA:

38 uL ddH2O
5 ul 10x expand buffer
5 ul 2mM dNTPs
0.33 ul o1
0.33 ul o2
o.33 ul o9
0.75 ul Expand polymerase