SBB12Ntbk-Hyungjun Kim
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Hyungjun Kim March 2st
-All the colonies I picked for sbb1203 didn't grow...big surprise.
-Zach picked 2 sbb1124 colonies for me and they grew. I finished the miniprep on that. I really hope these don't run blank on gels like last time.
-I also took my products from the PCA amp step for sbb1224 and sbb1203 and digested them. I'm starting over from this step.
Hyungjun Kim March 2st
-The plate for sbb1203 actually looked like E.Coli so that's a good sign. Went ahead and picked colonies. Keeping fingers crossed on this.
-I did a ligation and transformation on the sbb1224 digest part and the bca1834 vector digest.
-Plated the sbb 1224 transformation product, hopefully it's better or I'll have to go back a step further.
Hyungjun Kim March 1st
-None of the colonies grew for sbb 1203, so I went ahead to do another transformation and plated.
-I digested the sbb1224 minipreps and ran them on a analytical gel
-Well that was depressing, nothing came out on the DNA gel for sbb1224, so I guess I'll backtrack to the digested 1224 part and do a digestion/transformation again.
Hyungjun Kim February 28th
-Picked up the cell cultures that had been picked over the weekend by Zach
-The 3 tubes for sbb1224 looked good for mini preps while one dubious tube looked okay for sbb1203
-I went ahead and mini-prepped the 4 tubes.
-I also picked 4 more colonies for sbb 1203, hopefully these turn out fine
Hyungjun Kim February 24th
-Ran zymo cleanup on for the sbb1224 gel purification -Performed transformation with sbb1224 and sbb1224 into competent cells
Hyungjun Kim February 23th
-Ran zymo cleanups on the PCA amplification products -Did a NheI/BamI digest -I proceeded with a zymo on sbb1203 and did a gel purification on sbb 1224
Hyungjun Kim February 21th
-I ran zymo cleanups on the PCA products for sbb 1203 and 1224. -Small fragment cleanup was used for sbb1203 and regular zymo cleanup for 1224. -I set up the 2nd PCA amplification step for both parts.
Hyungjun Kim February 16th
-Started on making sbb1203 and sbb1224 parts -Ran the first PCA steps for sbb1203 and sbb1224
Hyungjun Kim
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