SBB12Ntbk-DanielChao: Difference between revisions
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==[[User:Daniel Chao|Daniel]] 12:30, 21 February 2012 (PST)== | ==[[User:Daniel Chao|Daniel]] 12:30, 21 February 2012 (PST)== | ||
Ran gel of two PCR products used for SOEing [[SBB12_gels | Gel pics]] <br> | Ran gel of two PCR products used for SOEing [[SBB12_gels | Gel pics]] <br>. Gel #1, lanes 3 and 4 pertain to fragments A and B in the construction file for ssb1221. | ||
Cut out gel and purified using [[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br> | Cut out gel and purified using [[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br> | ||
Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup. | Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup. | ||
Revision as of 13:37, 21 February 2012
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Daniel 11:30, 16 February 2012 (PST)
Prepared PCR reactions for sb1221:
PCR ca998/gfRevPR on pBjk2741-jtk2801 (160bp, A)
Placed in 100-500 PCR tray
PCR dc5002/g00101 on pBgl00001-Brp0006 (1100bp, B)
Placed in 1K-2K PCR tray
Using protocol as described here: Cloning by PCR
Prepared Wobble reaction for sbb1228
Wobble dc5003/dc5004 (64bp, wobpdt)
Using protocol as described here: Wobble Reaction
Daniel 14:00, 17 February 2012 (PST)
Performed short-fragment Zymo on wobble product, using protocol as described here Small-Frag Zymo Cleanup
Daniel 12:30, 21 February 2012 (PST)
Ran gel of two PCR products used for SOEing Gel pics
. Gel #1, lanes 3 and 4 pertain to fragments A and B in the construction file for ssb1221.
Cut out gel and purified using Zymo Gel Purification
Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup.
