SBB12Ntbk-DanielChao: Difference between revisions
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Prepared PCR reactions for sb1221: | Prepared PCR reactions for sb1221: | ||
PCR ca998/gfRevPR on pBjk2741-jtk2801 (160bp, A) | PCR ca998/gfRevPR on pBjk2741-jtk2801 (160bp, A) <br> | ||
Placed in 100-500 PCR tray | Placed in 100-500 PCR tray <br> | ||
PCR dc5002/g00101 on pBgl00001-Brp0006 (1100bp, B) | PCR dc5002/g00101 on pBgl00001-Brp0006 (1100bp, B) <br> | ||
Placed in 1K-2K PCR tray | Placed in 1K-2K PCR tray <br> | ||
Using protocol as described here: [[Template:SBB-Protocols_PCR2 | Cloning by PCR]] | Using protocol as described here: [[Template:SBB-Protocols_PCR2 | Cloning by PCR]] | ||
Revision as of 12:38, 16 February 2012
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Daniel 11:30, 16 February 2012 (PST)
Prepared PCR reactions for sb1221:
PCR ca998/gfRevPR on pBjk2741-jtk2801 (160bp, A)
Placed in 100-500 PCR tray
PCR dc5002/g00101 on pBgl00001-Brp0006 (1100bp, B)
Placed in 1K-2K PCR tray
Using protocol as described here: Cloning by PCR
Prepared Wobble reaction for sbb1228
Wobble dc5003/dc5004 (64bp, wobpdt)
Using protocol as described here: Wobble Reaction
