SBB12Ntbk-DanielChao: Difference between revisions
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Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup. | Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup. | ||
==[[User:Daniel Chao|Daniel]] 12:30, 23 February 2012 (PST)== | |||
Set up final PCR reaction for SOEing. | |||
Performed ligation reaction for sb-1228 Wobble products. |
Revision as of 11:34, 23 February 2012
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Daniel 11:30, 16 February 2012 (PST)
Prepared PCR reactions for sb1221:
PCR ca998/gfRevPR on pBjk2741-jtk2801 (160bp, A)
Placed in 100-500 PCR tray
PCR dc5002/g00101 on pBgl00001-Brp0006 (1100bp, B)
Placed in 1K-2K PCR tray
Using protocol as described here: Cloning by PCR
Prepared Wobble reaction for sbb1228
Wobble dc5003/dc5004 (64bp, wobpdt)
Using protocol as described here: Wobble Reaction
Daniel 14:00, 17 February 2012 (PST)
Performed short-fragment Zymo on wobble product, using protocol as described here Small-Frag Zymo Cleanup
Daniel 12:30, 21 February 2012 (PST)
Ran gel of two PCR products used for SOEing Gel pics
Gel #1, lanes 3 and 4 pertain to fragments A (160bp) and B (1100bp) in the construction file for ssb1221.
Cut out gel and purified using Zymo Gel Purification
Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup.
Daniel 12:30, 23 February 2012 (PST)
Set up final PCR reaction for SOEing.
Performed ligation reaction for sb-1228 Wobble products.