SBB12Ntbk-ChrisAnderson

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Current revision (11:36, 13 March 2013) (view source)
(JCAnderson 11:34, 13 March 2013 (EDT))
 
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==[[User:JCAnderson|JCAnderson]] 11:34, 13 March 2013 (EDT)==
==[[User:JCAnderson|JCAnderson]] 11:34, 13 March 2013 (EDT)==
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EIPCR primers for making a 6xHis_tADH1 "Part 4" part plasmid
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sbb13-1F: GCATCGTCTCACATCATCATCATCATCACtaactcgaggcgaatttc
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sbb13-2R: ATGCCGTCTCAGATGggatggagaccgactacc
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template: pGG062
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PCR product should be 1947bp.  Cut with BsmBI, ligate, plate on LB+Cam (ColE1 origin).
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Then when you assemble an expression plasmid, you can either use pGG062 to add the stop codon+terminator, or this new plasmid to add a 6xHis+stop+terminator.
==[[User:JCAnderson|JCAnderson]] 22:16, 31 January 2012 (EST)==
==[[User:JCAnderson|JCAnderson]] 22:16, 31 January 2012 (EST)==
This is my next entry.
This is my next entry.

Current revision

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JCAnderson 11:34, 13 March 2013 (EDT)

EIPCR primers for making a 6xHis_tADH1 "Part 4" part plasmid

sbb13-1F: GCATCGTCTCACATCATCATCATCATCACtaactcgaggcgaatttc sbb13-2R: ATGCCGTCTCAGATGggatggagaccgactacc template: pGG062

PCR product should be 1947bp. Cut with BsmBI, ligate, plate on LB+Cam (ColE1 origin). Then when you assemble an expression plasmid, you can either use pGG062 to add the stop codon+terminator, or this new plasmid to add a 6xHis+stop+terminator.

JCAnderson 22:16, 31 January 2012 (EST)

This is my next entry.

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