SBB12Ntbk-Annie Joseph
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Annie Joseph 16:23, 13 February 2012 (EST)
Annie Joseph 12:30, 17 February 2012 (EST)
We did PCR today! ==For part sbb1216: Obtained and made a 10 micro molar solution of oligos ca998/osbb1333R and osbb1216F/g00101 for PCR. Set up two solutions for PCR:
This one I call A in construction file: 24uL ddH2O 3.3uL 10x Expand Buffer "2" 3.3uL dNTPs (2mM in each) 1uL ca998, 10uM 1uL osbb1333R, 10uM 0.5uL Expand polymerase "1" 0.5uL pBca9145-jtk2768
And... This one is B: 24uL ddH2O 3.3uL 10x Expand Buffer "2" 3.3uL dNTPs (2mM in each) 1uL osbb1216F, 10uM 1uL g00101, 10uM 0.5uL Expand polymerase "1" 0.5uL pBjk2741-jtk3346
==For part sbb1205 I set up a solution based on the PCA protocol on open wet ware.
