SBB11Ntbk-Xin Xin Lin: Difference between revisions
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== | ==[[User:Xin Xin Lin|Xin Xin Lin]] 15:01, 18 February 2011 (EST)== | ||
Work done on 2/16/11: | |||
Run Analytical Gel of PCR Product | |||
2uL PCR Product in 5uL Loading Buffer | |||
Load 5uL DNA Ladder & 8uL PCR Samples- Run at 180V | |||
Upload Gel Image- fadR, cspE, & cspG in lanes 4,5,&6 of Analytical Gel Image 1 | |||
PCR Products Visible | |||
Regular Zymo Cleanup- Followed Protcol | |||
Eluted Cleaned DNA in 33uL ddH2O- Stored in 140L BoxA | |||
==[[User:Xin Xin Lin|Xin Xin Lin]] 14:07, 15 February 2011 (EST)== | ==[[User:Xin Xin Lin|Xin Xin Lin]] 14:07, 15 February 2011 (EST)== |
Revision as of 13:01, 18 February 2011
Xin Xin Lin 15:01, 18 February 2011 (EST)
Work done on 2/16/11:
Run Analytical Gel of PCR Product
2uL PCR Product in 5uL Loading Buffer
Load 5uL DNA Ladder & 8uL PCR Samples- Run at 180V
Upload Gel Image- fadR, cspE, & cspG in lanes 4,5,&6 of Analytical Gel Image 1
PCR Products Visible
Regular Zymo Cleanup- Followed Protcol
Eluted Cleaned DNA in 33uL ddH2O- Stored in 140L BoxA
Xin Xin Lin 14:07, 15 February 2011 (EST)
PCR with P_fadR, P_cspE, & P_cspG
Diluted fadR-F & fadR-R Oligos with 243 and 298uL ddH2O to 100uM
Made 1:10 Dilution- 1uL 100uM Oligos in 9uL ddH2O to 10uM Dilution
P_cspE & P_cspG Oligos already diluted to 10uM- ss39f&r for P_cspE and ss40f&r for P_cspG
Used E.coli MG1655 Genomic DNA as Template- Used Expand Buffer & Expand Polymerase 1
PCR Products should be 767, 432, & 432bp respectively- Used 2K55 PCR Program because PCR Products<2000bp