SBB11Ntbk-SuhaniVora: Difference between revisions
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==[[User:Suhani Vora|Suhani Vora]] 14:08, 15 February 2011 (EST)== | ==[[User:Suhani Vora|Suhani Vora]] 14:08, 15 February 2011 (EST)== | ||
Today we set up PCR reactions for our promoter parts. | Today we set up PCR reactions for our promoter parts. <br> | ||
-Resuspended oligos PmalK_R and SV_06 to 100 um. <br> | |||
-Resuspended oligos PmalK_R and SV_06 to 100 um. | -Diluted primers to 10 um. <br> | ||
-Set up PCR for cloning using Expand Polymerase <br> | |||
-Diluted primers to 10 um. | 1. P_malk <br> | ||
2. P_nlpA <br> | |||
-Set up PCR for cloning using Expand Polymerase | 3. P_rfaQ <br> | ||
Thermocycler Program: 2K55 <br> | |||
1. P_malk | |||
2. P_nlpA | |||
3. P_rfaQ | |||
Thermocycler Program: 2K55 | |||
Revision as of 12:09, 15 February 2011
~~!~~
Suhani Vora 14:08, 15 February 2011 (EST)
Today we set up PCR reactions for our promoter parts.
-Resuspended oligos PmalK_R and SV_06 to 100 um.
-Diluted primers to 10 um.
-Set up PCR for cloning using Expand Polymerase
1. P_malk
2. P_nlpA
3. P_rfaQ
Thermocycler Program: 2K55
