SBB11Ntbk-MaryWang: Difference between revisions
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(New page: ==~~~~== PCR ss45r/ss45f on MG1655 genome. Used Expand polymerase because it was a genome. Put template DNA into PCR tube first then the Expand polymerase second. Did not dilute the oligos...) |
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==[[User:Mary Wang|Mary Wang]] 14:16, 15 February 2011 (EST)== | ==[[User:Mary Wang|Mary Wang]] 14:16, 15 February 2011 (EST)== | ||
PCR ss45r/ss45f on MG1655 genome. | PCR ss45r/ss45f on MG1655 genome. | ||
Used Expand polymerase because it was a genome. | Used Expand polymerase because it was a genome. | ||
Put template DNA into PCR tube first then the Expand polymerase second. | Put template DNA into PCR tube first then the Expand polymerase second. | ||
Did not dilute the oligos. | Did not dilute the oligos. | ||
Used cloning by PCR- basic PCR method., | Used cloning by PCR- basic PCR method. | ||
Amounts used: | |||
24uL ddH2O, | |||
3.3uL 10X expand buffer, | |||
3.3uL dNTP, | |||
1uL Oligo 1, | |||
1uL Oligo 2, | |||
0.5 Expand polymerase 1, | |||
0.5uL Template DNA | |||
Revision as of 12:00, 17 February 2011
Mary Wang 14:16, 15 February 2011 (EST)
PCR ss45r/ss45f on MG1655 genome.
Used Expand polymerase because it was a genome.
Put template DNA into PCR tube first then the Expand polymerase second.
Did not dilute the oligos.
Used cloning by PCR- basic PCR method.
Amounts used: 24uL ddH2O, 3.3uL 10X expand buffer, 3.3uL dNTP, 1uL Oligo 1, 1uL Oligo 2, 0.5 Expand polymerase 1, 0.5uL Template DNA
