SBB11Ntbk-M.A.N.G.

BE 140L Spring 2011 Team MANG Goal: Evaluate induction of stress by toxic genes in the promoter set for toxR. In essence, put Pbad-toxR in the cell, put a stress promoter-GFP in the cell, add arabinose until it starts getting toxic, measure the GFP on a plate reader (the Tecan).

Schedule Mon: Marcus comes in at 9am transforms ToxR plasmid into MC1061 compitent cells. Plate on SPEC. Tuesday: Nikit comes in the morning to pick a colony and culture it in 2ml LB. Wed: Gary comes in at 3:30pm. He dilutes the saturated 2ml culture into a flask full of 18ml LB. Measures OD every 45min. Wed: Averee comes in at 5pm and takes over. When OD=.5, she performs small scale competence protocol. After resuspending in TSS (90ul x , freeze the cells in -80.