SBB11Ntbk-Justin Wang: Difference between revisions

Revision as of 12:09, 22 February 2011

--Justin Wang 14:57, 7 February 2011 (EST)

First Post!

--Justin Wang 12:40, 15 February 2011 (EST)

Prepared my oligonucleotides for PCR.

   -followed protocol for sbb1110 and sbb1129
      -Oligos are aleady diluted to 10 microliters
      -setup pcr solutions to be places.

--Justin Wang 12:40, 17 February 2011 (EST)

PCR Product Failed. There was no volume in the pcr product.

Redid the PCR setup following protocol.

Set up the Eco/Bam digestion for the cut and paste process. jtk2259

    -Used thermocycler to start the digestion
    -ran analytical gel on the part. Cut out the large bane in geland purified with zynos cleanup

--Justin Wang 12:40, 18 February 2011 (EST)

Ran the Gel on the PCR products - sbb1110 and sbb1129

 -Did the gel electrophoresis protocol.
 -analyzed gel picture
 -sbb1110 failed. No bands need redo.

Zymos cleanup on the gel product of the jtk2259.

--Justin Wang 12:40, 18 February 2011 (EST)

Experiment previously used wrong AW buffer and the entire previous process was redone.

New gel pic

   sbb1110 lane 10
   sbb1129 lane 12

Digested the product

 sbb1110 gel D pos 2
 sbb1129 gel D pos 3

@@ Line 29: / Line 29: @@
       -Used thermocycler to start the digestion
       -ran analytical gel on the part. Cut out the large bane in geland purified with zynos cleanup
+--[[User:Justin Wang|Justin Wang]] 12:40, 18 February 2011 (EST)
+<h2> Entry 2 </h2>
+Ran the Gel on the PCR products - sbb1110 and sbb1129
+  -Did the gel electrophoresis protocol.
+  -analyzed gel picture
+  -sbb1110 failed. No bands need redo.
+Zymos cleanup on the gel product of the jtk2259.
+--[[User:Justin Wang|Justin Wang]] 12:40, 18 February 2011 (EST)
+<h2> Entry 2 </h2>
+Experiment previously used wrong AW buffer and the entire previous process was redone.
+New gel pic[[Image:022111-AnalGel3.jpg]]
+    sbb1110 lane 10
+    sbb1129 lane 12
+Digested the product
+  sbb1110 gel D pos 2
+  sbb1129 gel D pos 3