SBB11Ntbk-Justin Wang: Difference between revisions
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Prepared my oligonucleotides for PCR. | Prepared my oligonucleotides for PCR. | ||
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--[[User:Justin Wang|Justin Wang]] 12:40, 17 February 2011 (EST) | --[[User:Justin Wang|Justin Wang]] 12:40, 17 February 2011 (EST) | ||
< | <h2> Entry 2 </h2> | ||
<i>PCR Product Failed. There was no volume in the pcr product.</i> | <i>PCR Product Failed. There was no volume in the pcr product.</i> | ||
Revision as of 11:58, 22 February 2011
--Justin Wang 14:57, 7 February 2011 (EST)
First Post!
--Justin Wang 12:40, 15 February 2011 (EST)
Entry 1
Prepared my oligonucleotides for PCR.
-followed protocol for sbb1110 and sbb1129
-Oligos are aleady diluted to 10 microliters
-setup pcr solutions to be places.
--Justin Wang 12:40, 17 February 2011 (EST)
Entry 2
PCR Product Failed. There was no volume in the pcr product.
Redid the PCR setup following protocol.
Set up the Eco/Bam digestion for the cut and paste process. jtk2259
-Used thermocycler to start the digestion
-ran analytical gel on the part. Cut out the large bane in geland purified with zynos cleanup
