SBB11Ntbk-Justin Wang: Difference between revisions
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First Post! | First Post! | ||
--[[User:Justin Wang|Justin Wang]] 12:40, 15 February 2011 (EST) | |||
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<b> Entry 1 </b> | |||
Prepared my oligonucleotides for PCR. | |||
-followed protocol for sbb1110 and sbb1129 | |||
-Oligos are aleady diluted to 10 microliters | |||
-setup pcr solutions to be places. | |||
--[[User:Justin Wang|Justin Wang]] 12:40, 17 February 2011 (EST) | |||
<b> Entry 2 </b> | |||
<i>PCR Product Failed. There was no volume in the pcr product.</i> | |||
Redid the PCR setup following protocol. | |||
Set up the Eco/Bam digestion for the cut and paste process. jtk2259 | |||
-Used thermocycler to start the digestion | |||
-ran analytical gel on the part. Cut out the large bane in geland purified with zynos cleanup |
Revision as of 11:56, 22 February 2011
--Justin Wang 14:57, 7 February 2011 (EST)
First Post!
--Justin Wang 12:40, 15 February 2011 (EST)
Entry 1
Prepared my oligonucleotides for PCR.
-followed protocol for sbb1110 and sbb1129 -Oligos are aleady diluted to 10 microliters -setup pcr solutions to be places.
--Justin Wang 12:40, 17 February 2011 (EST)
Entry 2
PCR Product Failed. There was no volume in the pcr product.
Redid the PCR setup following protocol.
Set up the Eco/Bam digestion for the cut and paste process. jtk2259
-Used thermocycler to start the digestion -ran analytical gel on the part. Cut out the large bane in geland purified with zynos cleanup