SBB11Ntbk-James Macaulay
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jamesmmacaulay@gmail.com 13:23, 15 February 2011 (EST)
- Set up PCR for part sbb1142 with 2K55 protocol.
jamesmmacaulay@gmail.com 13:44, 17 February 2011 (EST)
- Set up an analytical gel for part sbb1142, which I renamed as jm54 to differentiate from the mass of other parts with the sbb prefix.
- No visible bands showed up, need to set up new PCR.
- New PCR setup:
- jm54-W
- PCR_Protocol
- jm54-D
- PCR_Protocol -3.3µL ddH20 + 3.3µL DMSO.
- Submit to 2k45 protocol.
- New PCR setup:
- No visible bands showed up, need to set up new PCR.
- Set up a digest of pBca9145-jtk2979 with EcoRI/BamHI.
- Used 5µL of PCR product to be digested instead of 8µL.
