SBB11Ntbk-James Macaulay: Difference between revisions
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*** New PCR setup: | *** New PCR setup: | ||
***;jm54-W | ***;jm54-W | ||
***: [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 PCR_Protocol] | ***: [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 PCR_Protocol] -- '''2k45 PCR cycle''' | ||
***;jm54-D | ***;jm54-D | ||
***: [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 PCR_Protocol] -3.3µL ddH20 + 3.3µL DMSO. | ***: [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 PCR_Protocol] -3.3µL ddH20 + 3.3µL DMSO. -- '''2k45 PCR cycle''' | ||
* Set up a [http://openwetware.org/wiki/Template:SBB-Protocols_Enz2 digest] of pBca9145-jtk2979 with EcoRI/BamHI. | * Set up a [http://openwetware.org/wiki/Template:SBB-Protocols_Enz2 digest] of pBca9145-jtk2979 with EcoRI/BamHI. | ||
** Used 5µL of PCR product to be digested instead of 8µL. | ** Used 5µL of PCR product to be digested instead of 8µL. | ||
Revision as of 13:47, 17 February 2011
jamesmmacaulay@gmail.com 13:23, 15 February 2011 (EST)
- Set up PCR for part sbb1142 with 2K55 protocol.
jamesmmacaulay@gmail.com 13:44, 17 February 2011 (EST)
- Set up an analytical gel for part sbb1142, which I renamed as jm54 to differentiate from the mass of other parts with the sbb prefix.
- No visible bands showed up, need to set up new PCR.
- New PCR setup:
- jm54-W
- PCR_Protocol -- 2k45 PCR cycle
- jm54-D
- PCR_Protocol -3.3µL ddH20 + 3.3µL DMSO. -- 2k45 PCR cycle
- New PCR setup:
- No visible bands showed up, need to set up new PCR.
- Set up a digest of pBca9145-jtk2979 with EcoRI/BamHI.
- Used 5µL of PCR product to be digested instead of 8µL.
