SBB11Ntbk-Gary Dixon

Gary Dixon 14:32, 15 February 2011 (EST)

Performing 3 PCR reactions. One for part sbb1117, and two for part sbb1127 (soeing).

First, sorted out some confusion about letter R and F on oligo. R and F don't necessarily correspond to your forward and reverse oligo. So ss53r is the forward oligo for P_nmpC and ss53f is the reverse.

Then diluted oligos gd031 and gd032.

Then went through the standard procedure to set up PCR reactions. I have three in total.

ss53r/gd031R (718bp, gp = A)

gd032F/ss53f (287bp, gp = B)

ss42r/ss42f (1032bp)

The procedure: http://openwetware.org/wiki/Template:SBB-Protocols_PCR2

Put all three PCR reactions in the <2,000kb reaction.

Gary Dixon 23:59, 5 February 2011 (EST)

Hello World