SBB11Ntbk-Averee Chang: Difference between revisions
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==[[User:Averee Chang| Averee Chang]] 13:03, 3 February 2011 (EST)== | ==[[User:Averee Chang| Averee Chang]] 13:03, 3 February 2011 (EST)== | ||
Tuesday (February 15): Did a PCR to make P_narP from oligos ss34F, ss34R and P_sfmC from oligos ss52F,ss52R. Today, my PCR yield had too little volume (probably due to a loose cap on the PCR tube) and I re-did the PCR for both of them today | Tuesday (February 15): Did a PCR to make P_narP from oligos ss34F, ss34R and P_sfmC from oligos ss52F,ss52R. Today, my PCR yield had too little volume (probably due to a loose cap on the PCR tube) and I re-did the PCR for both of them today using protocol <br> | ||
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Today, along with re-doing the two PCRs, I digested part jtk2914 from pBjh1601KC-jtk2914 using EcoRI and BamHI. I | Today, along with re-doing the two PCRs, I digested part jtk2914 from pBjh1601KC-jtk2914 using EcoRI and BamHI. After doing a preparation gel, I cut out the larger band of the two and melted the agarose gel and froze it in box "C." I digested with EcoRI/BamHI and underwent the preparation gel using [[Template:SBB-Protocols Enz2 | EcoRI/BamHI Digest of PCR Products]] | ||
Revision as of 13:50, 17 February 2011
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Averee Chang 13:03, 3 February 2011 (EST)
Tuesday (February 15): Did a PCR to make P_narP from oligos ss34F, ss34R and P_sfmC from oligos ss52F,ss52R. Today, my PCR yield had too little volume (probably due to a loose cap on the PCR tube) and I re-did the PCR for both of them today using protocol
Today, along with re-doing the two PCRs, I digested part jtk2914 from pBjh1601KC-jtk2914 using EcoRI and BamHI. After doing a preparation gel, I cut out the larger band of the two and melted the agarose gel and froze it in box "C." I digested with EcoRI/BamHI and underwent the preparation gel using EcoRI/BamHI Digest of PCR Products
