SBB11Ntbk-Averee Chang: Difference between revisions
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[[Template:SBB11Projectstdt1118 | To My Project Page]]<br> | [[Template:SBB11Projectstdt1118 | To My Project Page]]<br> | ||
[[Template:SBB11Indiv-1113 | To Averee's Personal Page]]<br> | [[Template:SBB11Indiv-1113 | To Averee's Personal Page]]<br> | ||
[[SynBioBootcamp:Notebook/SynBioBootcamp11 | To | [[SynBioBootcamp:Notebook/SynBioBootcamp11 | To SynBioBootcamp Homepage]]<br> | ||
==[[User:Averee Chang| Averee Chang]] 13:03, 3 February 2011 (EST)== | ==[[User:Averee Chang| Averee Chang]] 13:03, 3 February 2011 (EST)== | ||
Revision as of 12:16, 17 February 2011
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Averee Chang 13:03, 3 February 2011 (EST)
Tuesday (February 15): Did a PCR to make P_narP from oligos ss34F, ss34R and P_sfmC from oligos ss52F,ss52R. Today, my PCR yield had too little volume (probably due to a loose cap on the PCR tube) and I re-did the PCR for both of them today.
Today, along with re-doing the two PCRs, I digested part jtk2914 from pBjh1601KC-jtk2914 using EcoRI and BamHI. I will sub this into pBca9523-Bca114#5. The product should be pBca9523-jtk2914
