SBB11Ntbk-Averee Chang: Difference between revisions
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Revision as of 12:11, 17 February 2011
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Averee Chang 13:03, 3 February 2011 (EST)
Tuesday (February 15): Did a PCR to make P_narP from oligos ss34F, ss34R and P_sfmC from oligos ss52F,ss52R. Today, my PCR yield had too little volume (probably due to a loose cap on the PCR tube) and I re-did the PCR for both of them today.
Today, along with re-doing the two PCRs, I digested part jtk2914 from pBjh1601KC-jtk2914 using EcoRI and BamHI. I will sub this into pBca9523-Bca114#5. The product should be pBca9523-jtk2914
