SBB11Ntbk-Anand Kesavaraju

User:Anand Kesavaraju 17 February 2011 (PST)

• Repeat sbb1140 (BseRImet) PCR - did not work last time.
• Run analytical gel for both (just P_malP for today)

User:Anand Kesavaraju 15 February 2011 (PST)

PCR of sbb1140 and sbb1107

• Only PCR today
• Oligos sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM
• sbb1107F/R are already at 10 uM

• Expand buffer/polymerase for genomic (MG1655 DNA)
• Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009)

Followed the following Protocol for Cloning by PCR.

User:Anand Kesavaraju 11 February 2011 (PST)

I herd u liek notebooks, so I put a notebook in ur notebook so you can look at Anand's Personal Page