SBB11Ntbk-Anand Kesavaraju: Difference between revisions
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==Welcome to Anand Kesavaraju's 140L Lab Notebook == | |||
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==[[User:Anand Kesavaraju]] 14:24, 15 February 2011 (EST)== | ==[[User:Anand Kesavaraju]] 14:24, 15 February 2011 (EST)== | ||
PCR of sbb1140 and sbb1107 | PCR of sbb1140 and sbb1107 | ||
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* Expand buffer/polymerase for genomic (MG1655 DNA) | * Expand buffer/polymerase for genomic (MG1655 DNA) | ||
* Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009) | * Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009) | ||
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Followed the following [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 Protocol for Cloning by PCR]. | Followed the following [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 Protocol for Cloning by PCR]. | ||
==[[User:Anand Kesavaraju|anand.k@berkeley.edu]] 14:24, 11 February 2011 (EST)== | ==[[User:Anand Kesavaraju|anand.k@berkeley.edu]] 14:24, 11 February 2011 (EST)== | ||
I herd u liek notebooks, so I put a notebook | I herd u liek notebooks, so I put a notebook in ur notebook so you can look at [http://openwetware.org/wiki/User:Anand_Kesavaraju Anand's Personal Page] | ||
[http://openwetware.org/wiki/User:Anand_Kesavaraju Anand's Personal Page] | |||
Revision as of 18:51, 15 February 2011
Welcome to Anand Kesavaraju's 140L Lab Notebook
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User:Anand Kesavaraju 14:24, 15 February 2011 (EST)
PCR of sbb1140 and sbb1107
- Only PCR today
- Oligos sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM
- sbb1107F/R are already at 10 uM
- Expand buffer/polymerase for genomic (MG1655 DNA)
- Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009)
Followed the following Protocol for Cloning by PCR.
anand.k@berkeley.edu 14:24, 11 February 2011 (EST)
I herd u liek notebooks, so I put a notebook in ur notebook so you can look at Anand's Personal Page
