SBB11Ntbk-Anand Kesavaraju: Difference between revisions
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==Welcome to Anand Kesavaraju's 140L Lab Notebook == | ==Welcome to Anand Kesavaraju's 140L Lab Notebook == | ||
==[[User:Anand Kesavaraju]] 15 February 2011 ( | ==[[User:Anand Kesavaraju]] 17 February 2011 (PST)== | ||
* Repeat sbb1140 (BseRImet) PCR - did not work last time. | |||
* Run analytical gel for both (just P_malP for today) | |||
==[[User:Anand Kesavaraju]] 15 February 2011 (PST)== | |||
PCR of sbb1140 and sbb1107 | PCR of sbb1140 and sbb1107 | ||
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Followed the following [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 Protocol for Cloning by PCR]. | Followed the following [http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 Protocol for Cloning by PCR]. | ||
==[[User:Anand Kesavaraju]] 11 February 2011 (EST)== | ==[[User:Anand Kesavaraju]] 11 February 2011 (EST)== | ||
I herd u liek notebooks, so I put a notebook in ur notebook so you can look at [http://openwetware.org/wiki/User:Anand_Kesavaraju Anand's Personal Page] | I herd u liek notebooks, so I put a notebook in ur notebook so you can look at [http://openwetware.org/wiki/User:Anand_Kesavaraju Anand's Personal Page] | ||
Revision as of 11:55, 17 February 2011
Welcome to Anand Kesavaraju's 140L Lab Notebook
User:Anand Kesavaraju 17 February 2011 (PST)
- Repeat sbb1140 (BseRImet) PCR - did not work last time.
- Run analytical gel for both (just P_malP for today)
User:Anand Kesavaraju 15 February 2011 (PST)
PCR of sbb1140 and sbb1107
- Only PCR today
- Oligos sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM
- sbb1107F/R are already at 10 uM
- Expand buffer/polymerase for genomic (MG1655 DNA)
- Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009)
Followed the following Protocol for Cloning by PCR.
User:Anand Kesavaraju 11 February 2011 (EST)
I herd u liek notebooks, so I put a notebook in ur notebook so you can look at Anand's Personal Page
