SBB11Ntbk-Anand Kesavaraju: Difference between revisions
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* Only PCR today | * Only PCR today | ||
* sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM | * Oligos sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM | ||
* sbb1107F/R are already at 10 uM | * sbb1107F/R are already at 10 uM | ||
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* Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009) | * Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009) | ||
[http://openwetware.org/wiki/Template:SBB-Protocols_PCR2 Protocol for Cloning by PCR] | |||
</blockquote> | |||
--[[User:Anand Kesavaraju|anand.k@berkeley.edu]] 14:24, 11 February 2011 (EST) | |||
I herd u liek notebooks, so I put a notebook.... | |||
[http://openwetware.org/wiki/User:Anand_Kesavaraju Anand's Personal Page] | [http://openwetware.org/wiki/User:Anand_Kesavaraju Anand's Personal Page] | ||
Revision as of 18:46, 15 February 2011
Welcome to Anand Kesavaraju's 140L Lab Notebook
--anand.k@berkeley.edu 14:24, 15 February 2011 (EST) PCR of sbb1140 and sbb1107
- Only PCR today
- Oligos sbb1140F/R are freeze dried, so rehydrate with appropriate volume of ddH2O and perform a 1:10 dilution to get 10 uM
- sbb1107F/R are already at 10 uM
- Expand buffer/polymerase for genomic (MG1655 DNA)
- Phusion buffer/polymerase for plasmid DNA (pBjh1601CA-Bgl009)
--anand.k@berkeley.edu 14:24, 11 February 2011 (EST)
I herd u liek notebooks, so I put a notebook.... Anand's Personal Page
