SBB10 projects

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Revision as of 10:53, 31 January 2010 by JCAnderson (talk | contribs)
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Welcome to your project page!

For each part listed, you should:

  • Design oligos to make your part
  • Write up proper construction files and put it on the Construction Files page
  • Put your oligos on the Oligo Log page
  • Put your parts into Clotho

You should design your construction strategy to put your part into plasmid vectorName-Bca1144 (Where vectorName is indicated for each part) using EcoRI and BamHI or just BamHI or BglII for EIPCR. The maps for all plasmids involved in our project can be downloaded here.

Several references have been provided to give you some background on the biology of your part.

Also, use the link above left to upload a picture of yourself, your name, and anything else you'd like.

Finally, you should create a notebook on the main page of the wiki

09 notes
General notes


Families by part type:

Complete Gene part family
Complete Gene part family


Promoter and CDS, no terminator part family
Promoter and RNA, no terminator part family
Enzyme family
DNA binding domain family
cis element family
Rep protein family
Antibiotic marker family


Families by device grouping:

N15 Protelomerase family
piggyBac family
Tn5 family
Sleeping Beauty family
ISY100 family
phiC31 integration family
Zinc Finger Nuclease family
Zinc Finger Transposase family
Orthogonal Replicon family