SBB10 projects: Difference between revisions
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=== | ===Assay Teams=== | ||
[[Template: | [[Template:SBB10AssayTeam1 | Team 1: Toxicity and Expression]]<br> | ||
[[Template: | [[Template:SBB10AssayTeam2 | Team 2: Lysis and Transposition]]<br> | ||
[[Template: | [[Template:SBB10AssayTeam3 | Team 3: Origin stability and copy number]]<br> | ||
[[Template: | [[Template:SBB10AssayTeam4 | Team 4: Toxicity and Expression]]<br> | ||
[[Template: | [[Template:SBB10AssayTeam5 | Team 5: ZFN and phiC31 Activity]]<br> | ||
[[ | |||
[[Template:SBB10Project- | ===Composite Parts=== | ||
[[Template:SBB10Project- | [[Media:140L_parts1_032910.xls | Composite Parts List]] | ||
[[Template:SBB10Project- | |||
[[Template:SBB10Project- | ===Part Project Pages:=== | ||
[[Template:SBB10Project- | [[Template:SBB10Project-11097 | 11097-Zinc Finger Domain (zf+), Zinc Finger Domain (zf-)]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-11956 | 11956-piggieBac 5'TR, N15 Protelomerase]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-13567 | 13567-piggieBac 3'TR, N15 Protelomerase]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-14224 | 14224-tos site of N15, piggyBac transposase]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-15924 | 15924-sleeping beauty 5'TR, PhiC31 Integrase]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-15954 | 15954-sleeping beauty 3'TR, PhiC31 Integrase]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-18094 | 18094-FokI Cleavage Domain (fok-), P9 rep Protein]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-19244 | 19244-FokI Cleavage Domain (fok-), P9 origin of replication]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-19329 | 19329-Tn5 3'TR, Gentamicin resistance gene]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-19395 | 19395-Ala6 linker, Tn5 Transposase]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-19607 | 19607-FokI Cleavage Domain (fok+), Tn5 5'TR]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-20154 | 20154-FokI Cleavage Domain (fok+), Zinc Finger target]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-26253 | 26253-CA42 origin of replication, phiC31 attB]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-27095 | 27095-CA42 rep Protein, phiC31 attP]]<br> | ||
[[Template:SBB10Project- | [[Template:SBB10Project-28190 | 28190-O99 origin of replication, O99 regulatory RNA, Constitutive RFP]]<br> | ||
[[Template:SBB10Project-29071 | 29071-O99 rep Protein, CA42 rep Gene, Pre-Pro sequence]]<br> | |||
[[Template:SBB10Project-37613 | 37613-P9 regulatory RNA, CA42 regulatory RNA, Pre-Pro sequence]]<br> | |||
[[Template:SBB10Project-37738 | 37738-O99 rep Gene, Pbad promoter, Constitutive promoter]]<br> | |||
[[Template:SBB10Project-38352 | 38352-Sleeping beauty (SB100x), Magnesium-repressed Promoter, Nuclear localization peptide]]<br> | |||
===Families by part type:=== | ===Families by part type:=== | ||
| Line 50: | Line 54: | ||
===Experimental notes:=== | ===Experimental notes:=== | ||
[[Template:SBB10_RBSCalculator | Part requires RBS calculator]]<br> | [[Template:SBB10_RBSCalculator | Part requires RBS calculator]]<br> | ||
[[Template:SBB10_EcoBamXfer | Part requires an EcoRI/BamHI Transfer]]<br> | |||
[[Template:SBB10_GeneSynthesisRequired | Part requires Gene Synthesis]]<br> | |||
Latest revision as of 13:37, 5 April 2010
Assay Teams
Team 1: Toxicity and Expression
Team 2: Lysis and Transposition
Team 3: Origin stability and copy number
Team 4: Toxicity and Expression
Team 5: ZFN and phiC31 Activity
Composite Parts
Part Project Pages:
11097-Zinc Finger Domain (zf+), Zinc Finger Domain (zf-)
11956-piggieBac 5'TR, N15 Protelomerase
13567-piggieBac 3'TR, N15 Protelomerase
14224-tos site of N15, piggyBac transposase
15924-sleeping beauty 5'TR, PhiC31 Integrase
15954-sleeping beauty 3'TR, PhiC31 Integrase
18094-FokI Cleavage Domain (fok-), P9 rep Protein
19244-FokI Cleavage Domain (fok-), P9 origin of replication
19329-Tn5 3'TR, Gentamicin resistance gene
19395-Ala6 linker, Tn5 Transposase
19607-FokI Cleavage Domain (fok+), Tn5 5'TR
20154-FokI Cleavage Domain (fok+), Zinc Finger target
26253-CA42 origin of replication, phiC31 attB
27095-CA42 rep Protein, phiC31 attP
28190-O99 origin of replication, O99 regulatory RNA, Constitutive RFP
29071-O99 rep Protein, CA42 rep Gene, Pre-Pro sequence
37613-P9 regulatory RNA, CA42 regulatory RNA, Pre-Pro sequence
37738-O99 rep Gene, Pbad promoter, Constitutive promoter
38352-Sleeping beauty (SB100x), Magnesium-repressed Promoter, Nuclear localization peptide
Families by part type:
Complete Gene part family
Promoter and CDS, no terminator part family
Promoter and RNA, no terminator part family
Enzyme family
DNA binding domain family
cis element family
Rep protein family
Antibiotic marker family
Families by device grouping:
N15 Protelomerase family
piggyBac family
Tn5 family
Sleeping Beauty family
ISY100 family
phiC31 integration family
Zinc Finger Nuclease family
Zinc Finger Transposase family
Orthogonal Replicon family
Experimental notes:
Part requires RBS calculator
Part requires an EcoRI/BamHI Transfer
Part requires Gene Synthesis
