SBB10Ntbk-ZhenZongHuang: Difference between revisions
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==[[User:Zhen Zong Huang|Zhen Z. Huang]] 15:05, 16 February 2010 (EST)== | |||
===Planning for Wed, Feb 17th, 2010=== | |||
===sbb31=== | |||
1. PCR | |||
2. Analytucal gel | |||
3. Zymol clean up | |||
4. digestion | |||
5. gel purification | |||
6. ligation | |||
7. mapping | |||
8. gel purification | |||
===sbb15=== | |||
1. Wobble | |||
2. Analytucal gel | |||
3. Zymol small clean up | |||
4. digestion | |||
5. gel purification | |||
6. ligation | |||
7. mapping | |||
8. gel purification | |||
==[[User:Zhen Zong Huang|Zhen Z. Huang]] 13:00, 18 February 2010 (EST)== | |||
==Construction Files for my parts== | ==Construction Files for my parts== | ||
===sbb31=== | ===sbb31=== |
Revision as of 16:11, 16 February 2010
Zhen Z. Huang 15:05, 16 February 2010 (EST)
Planning for Wed, Feb 17th, 2010
sbb31
1. PCR 2. Analytucal gel 3. Zymol clean up 4. digestion 5. gel purification 6. ligation 7. mapping 8. gel purification
sbb15
1. Wobble 2. Analytucal gel 3. Zymol small clean up 4. digestion 5. gel purification 6. ligation 7. mapping 8. gel purification
Zhen Z. Huang 13:00, 18 February 2010 (EST)
Construction Files for my parts
sbb31
sbb31: CA42 origin of replication Source: pEC22-CA42 Target Sequence: agcacttcagcgcgccgtagcatcgataaacattacgggatggggcgaaactgccatctgttcgaaatgacgcgcaaatgggcttacagggcgattcgtcagggctggccagcattctcacagtggcttgatgccgtgattcagcgtgtcgaaatgtacaacgcatcgcttcccgttccgctttcacctcctgaatgtcgggctattggcaagagtattgcgaaatacacgcacaggaacttcacggcggaaactttcgcacagtatgtggctgatacgcacacgccagaaatacaggccaagagaggcaggaaaggtggcatcgctaaaggcgaagcctacgatgacaagcgtttcatggcgctatgtatgctggagaatggatattctcagaaagctattgcggcgatgttggaggtttctactcgaaccattcgaaactggaaaagcggaaaatagcctatatcagataacagcgcctttctggcgtttttttgagcagtaggtcttttgccg Vector: pBjk2741 Short description: oriCA42 Genbank reference: D30056.1 Family: Origin of Replication PCR ZZH001 and ZZH002 on pEC22-CA42 (547 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144 (EcoRI/BamHI, 2472+910, L) Product is pBca9523-sbb31 {oriCA42} ---------------------------- ZZH001 Forward oligo for cloning of oriCA42 ccataGAATTCatgAGATCTagcacttcagcgcgccgtag ZZh002 Reverse oligo for cloning of oriCA42 ctgatGGATCCcggcaaaagacctactgctc
sbb15
sbb15: phiC31 attB Source: Synthetic Target Sequence: tgacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgggctccccgggcgcgtactccacctcacccatctggtcca Vector: pBjk2741 Short descriptions: phiC31 attB Genbank reference: AB306970 (759…842) Family: Phage att site good 20 bp gtgccagggcgtgcccttgg Wobble ZZH003/ZZH004 (115bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb15 {phiC31 attB} ---- ZZH003 Forward construction of phiC31 attB basic part ccataGAATTCatgAGATCTtgacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgg ZZH004 Reverse construction of phiC31 attB basic part ctgatGGATCCtggaccagatgggtgaggtggagtacgcgcccggggagcccaagggcacgccctggcac