SBB10Ntbk-ZhenZongHuang: Difference between revisions
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(New page: ==Construction Files for my parts== ===sbb31=== sbb31: CA42 origin of replication Source: pEC22-CA42 Target Sequence: agcacttcagcgcgccgtagcatcgataaacattacgggatggggcgaaactgccatctgttcgaaat...) |
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==Construction Files for my parts== | ==Construction Files for my parts== | ||
===sbb31=== | ===sbb31=== | ||
<pre> | |||
sbb31: CA42 origin of replication | sbb31: CA42 origin of replication | ||
Source: pEC22-CA42 | Source: pEC22-CA42 | ||
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Genbank reference: D30056.1 | Genbank reference: D30056.1 | ||
Family: Origin of Replication | Family: Origin of Replication | ||
PCR ZZH001 and ZZH002 on pEC22-CA42 (547 bp, EcoRI/BamHI) | PCR ZZH001 and ZZH002 on pEC22-CA42 (547 bp, EcoRI/BamHI) | ||
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ZZh002 Reverse oligo for cloning of oriCA42 | ZZh002 Reverse oligo for cloning of oriCA42 | ||
ctgatGGATCCcggcaaaagacctactgctc | ctgatGGATCCcggcaaaagacctactgctc | ||
</pre> | |||
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<pre> | |||
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ZZH004 Reverse construction of phiC31 attB basic part | ZZH004 Reverse construction of phiC31 attB basic part | ||
ctgatGGATCCtggaccagatgggtgaggtggagtacgcgcccggggagcccaagggcacgccctggcac | ctgatGGATCCtggaccagatgggtgaggtggagtacgcgcccggggagcccaagggcacgccctggcac | ||
</pre> | |||
Revision as of 00:24, 10 February 2010
Construction Files for my parts
sbb31
sbb31: CA42 origin of replication
Source: pEC22-CA42
Target Sequence: agcacttcagcgcgccgtagcatcgataaacattacgggatggggcgaaactgccatctgttcgaaatgacgcgcaaatgggcttacagggcgattcgtcagggctggccagcattctcacagtggcttgatgccgtgattcagcgtgtcgaaatgtacaacgcatcgcttcccgttccgctttcacctcctgaatgtcgggctattggcaagagtattgcgaaatacacgcacaggaacttcacggcggaaactttcgcacagtatgtggctgatacgcacacgccagaaatacaggccaagagaggcaggaaaggtggcatcgctaaaggcgaagcctacgatgacaagcgtttcatggcgctatgtatgctggagaatggatattctcagaaagctattgcggcgatgttggaggtttctactcgaaccattcgaaactggaaaagcggaaaatagcctatatcagataacagcgcctttctggcgtttttttgagcagtaggtcttttgccg
Vector: pBjk2741
Short description: oriCA42
Genbank reference: D30056.1
Family: Origin of Replication
PCR ZZH001 and ZZH002 on pEC22-CA42 (547 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144 (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-sbb31 {oriCA42}
----------------------------
ZZH001 Forward oligo for cloning of oriCA42
ccataGAATTCatgAGATCTagcacttcagcgcgccgtag
ZZh002 Reverse oligo for cloning of oriCA42
ctgatGGATCCcggcaaaagacctactgctc
===sbb15===
sbb15: phiC31 attB
Source: Synthetic
Target Sequence: tgacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgggctccccgggcgcgtactccacctcacccatctggtcca
Vector: pBjk2741
Short descriptions: phiC31 attB
Genbank reference: AB306970 (759…842)
Family: Phage att site
good 20 bp gtgccagggcgtgcccttgg
Wobble ZZH003/ZZH004 (115bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 2170+910, L)
Product is pBjk2741-sbb15 {phiC31 attB}
----
ZZH003 Forward construction of phiC31 attB basic part
ccataGAATTCatgAGATCTtgacggtctcgaagccgcggtgcgggtgccagggcgtgcccttgg
ZZH004 Reverse construction of phiC31 attB basic part
ctgatGGATCCtggaccagatgggtgaggtggagtacgcgcccggggagcccaagggcacgccctggcac
