SBB10Ntbk-Paulina Tran: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Paulina Tran (talk | contribs) No edit summary |
Paulina Tran (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
==[[User:Paulina Tran|Paulina Tran]] 02:00, 9 February 2010 (EST)== | |||
===Construction file for sbb21=== | |||
<pre> | <pre> | ||
Construction of FokI+ cleavage domain basic part sbb21 | Construction of FokI+ cleavage domain basic part sbb21 | ||
| Line 14: | Line 15: | ||
fk0003 (R)Base Substitution FokI cggcgccattgcAattggttttatgg | fk0003 (R)Base Substitution FokI cggcgccattgcAattggttttatgg | ||
fk0002 Reverse BamHI for FokI gctagGGATCCaaaattgatctccccattgttg | fk0002 Reverse BamHI for FokI gctagGGATCCaaaattgatctccccattgttg | ||
<pre/> | <pre/> | ||
===Construction file for sbb08=== | |||
<pre> | |||
EIPCR tn0001/tn0002R on pBjk2741-Bca1144 (2230 bp, BglII) | EIPCR tn0001/tn0002R on pBjk2741-Bca1144 (2230 bp, BglII) | ||
Product is pBca9145-Btn0001 {Tn5 5’TR} | Product is pBca9145-Btn0001 {Tn5 5’TR} | ||
| Line 25: | Line 26: | ||
tn0002R Reverse BglII oligo for Tn5 EIPCR | tn0002R Reverse BglII oligo for Tn5 EIPCR | ||
CCAATAGATCTcatgaattcCACTTCAG | CCAATAGATCTcatgaattcCACTTCAG | ||
<pre/> | |||
Revision as of 02:47, 9 February 2010
Paulina Tran 02:00, 9 February 2010 (EST)
Construction file for sbb21
Construction of FokI+ cleavage domain basic part sbb21 PCR fk0001/fk0003 on FokI (505 bp, gp = A) PCR fk0004/fk0002 on FokI (141 bp, gp = B)
PCR fk0001/fk0002 on A+B (620 bp, EcoRI/BamHI) Digest pBjk2741-Bca1144 (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-ssb21 {<FokI+>}
fk0001 Forward EcoRI and BglII for FokI ccaaaGAATTCgtccAGATCTCAGCTGGTTaaatctgaactggaggag fk0004 (F)Base Substitution FokI ccataaaaccaatTgcaatggcgccg fk0003 (R)Base Substitution FokI cggcgccattgcAattggttttatgg fk0002 Reverse BamHI for FokI gctagGGATCCaaaattgatctccccattgttgConstruction file for sbb08
EIPCR tn0001/tn0002R on pBjk2741-Bca1144 (2230 bp, BglII) Product is pBca9145-Btn0001 {Tn5 5’TR} ------- tn0001 EIPCR construction of Tn5 part CCATAagatctGTCGACTGTCTCTTATACACATCTCAACCggatcctaaCTCGCTCCTCaggcttc tn0002R Reverse BglII oligo for Tn5 EIPCR CCAATAGATCTcatgaattcCACTTCAG
