SBB10Ntbk-Mesa: Difference between revisions
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[[Template:SBB10Project-27095|Project]] | [[Template:SBB10Project-27095|Project]] | ||
sbb27: CA42 rep Protein | sbb27: CA42 rep Protein | ||
PCR KRM001 and KRM002 on pEC22-CA42 (987 bp, EcoRI/BamHI) | PCR KRM001 and KRM002 on pEC22-CA42 (987 bp, EcoRI/BamHI) | ||
Revision as of 23:07, 8 February 2010
sbb27: CA42 rep Protein
PCR KRM001 and KRM002 on pEC22-CA42 (987 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144 (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-sbb27 {rbs.repCA42!}
----------------------------
KRM001 Forward oligo for cloning of rbs.repCA42!
ccataGAATTCatgAGATCTcccggagagaatcgtaacac
KRM002 Reverse oligo for cloning of rbs.repCA42!
ctgatGGATCCctattttccgcttttccag
sbb16: phiC31 attP
Wobble KRM003/KRM004 (115bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 2170+910, L)
Product is pBjk2741-sbb16 {phiC31 attP}
----
KRM003 Forward construction of phiC31 attP basic part
ccataGAATTCatgAGATCTagaagcggttttcgggagtagtgccccaactggggtaacctttgag
KRM004 Reverse construction of phiC31 attP basic part
ctgatGGATCCgtgtcatgtcggcgaccctacgcccccaactgagagaactcaaaggttaccccagttg
