SBB10Ntbk-JoseGutierrez: Difference between revisions
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(New page: =Construction Files= ==sbb02: N15 Protelomerase== <pre> Biobricking of O99 rep Gene PCR JG001-F and JG002-R on pEC52 (1235 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144 (Eco...) |
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=Construction Files= | =Construction Files= | ||
== | ==SBB30: %'UTR_rep009== | ||
<pre> | <pre> | ||
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</pre> | </pre> | ||
==SBB38: PBad Promoter== | |||
<pre> | <pre> | ||
Eco/Bam transfer pBjh1601CK-ig114 | Eco/Bam transfer pBjh1601CK-ig114 | ||
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</pre> | </pre> | ||
==SBB37: PCon Promoter== | |||
<pre> | <pre> | ||
Eco/Bam transfer pBca1100-Bca1152 | Eco/Bam transfer pBca1100-Bca1152 | ||
Revision as of 17:44, 15 April 2010
Construction Files
SBB30: %'UTR_rep009
Biobricking of O99 rep Gene
PCR JG001-F and JG002-R on pEC52 (1235 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144 (EcoRI/BamHI, 910+2472, L)
Product is pBca9523-sbb30 {5'UTR_repO99!}
----------------------------
JG001-F Cloning of O99 rep Gene
ccataGAATTCatgAGATCTaaaaacgattctgacgcattttttatg
JG002-R Cloning of O99 rep Gene
CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc
SBB38: PBad Promoter
Eco/Bam transfer pBjh1601CK-ig114
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-ig114 {AraC-Pbad}
SBB37: PCon Promoter
Eco/Bam transfer pBca1100-Bca1152
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bca1152 {Pcon-J23100}
