SBB10Ntbk-JoannaChen: Difference between revisions
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Joanna Chen (talk | contribs) (New page: =~~~~= Today was the first day of wet lab. I obtained the tubes containing the oligo mixes and set up the assembly portion of PCA for both of my parts (sbb17 and sbb18). I combined: ...) |
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2. 5 ul 10x expand buffer | 2. 5 ul 10x expand buffer | ||
3. 5 ul 2mM dNTPs | 3. 5 ul 2mM dNTPs | ||
4. 1 ul oligo mixture | 4. 1 ul oligo mixture | ||
5. 0.75 ul Expand polymerase | 5. 0.75 ul Expand polymerase | ||
PCA tubes are labeled sbb17 PCA and sbb18 PCA respectively, and placed into the ice bucket for PCA. | PCA tubes are labeled sbb17 PCA and sbb18 PCA respectively, and placed into the ice bucket for PCA. | ||
Revision as of 15:26, 17 February 2010
Joanna Chen 17:25, 17 February 2010 (EST)
Today was the first day of wet lab.
I obtained the tubes containing the oligo mixes and set up the assembly portion of PCA for both of my parts (sbb17 and sbb18). I combined:
1. 38 uL ddH2O 2. 5 ul 10x expand buffer 3. 5 ul 2mM dNTPs 4. 1 ul oligo mixture 5. 0.75 ul Expand polymerase
PCA tubes are labeled sbb17 PCA and sbb18 PCA respectively, and placed into the ice bucket for PCA.
