SBB10Ntbk-JennaKelleher: Difference between revisions
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[[SBB10Ntbk-JennaKelleher2| 2/17/2010]]<br> | [[SBB10Ntbk-JennaKelleher2| 2/17/2010]]<br> | ||
[[SBB10Ntbk-JennaKelleher3| 2/22/2010]]<br> | [[SBB10Ntbk-JennaKelleher3| 2/22/2010]]<br> | ||
[[SBB10Ntbk-JennaKelleher1| 2/10/2010]]<br> | |||
===Project Information=== | |||
[[Template:SBB10Project-29071 | 29071-O99 rep Protein, CA42 rep Gene, Pre-Pro sequence]]<br> | |||
<br> | |||
===Construction Files=== | |||
[[Template:SBB10_ConstructionFiles_JennaK_sbb29 | Jenna Kelleher 099 Rep Protein (sbb29)]]<br> | |||
[[Template:SBB10_ConstructionFiles_JennaK_sbb28 | Jenna Kelleher CA42 Rep Gene (sbb28)]]<br> | |||
[[Template:SBB10_ConstructionFiles_JennaK_sbb42 | Jenna Kelleher Pre-pro Sequence (sbb42)]]<br> | |||
<br> | |||
===Useful Protocols=== | |||
For SBB28 and SBB29: <br> | |||
[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | |||
[[Template:SBB-Protocols_Zymo1 | Regular Zymo Cleanup]]<br> | |||
<br> | |||
For SBB42: <br> | |||
[[Template:SBB-EcoBamXfers | Eco-Bam Transfer]]<br> | |||
[[Template:SBB-Protocols_Zymo2 | Small-Frag Zymo Cleanup]]<br> | |||
<br> | |||
For All 3:<br> | |||
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br> | |||
[[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br> | |||
[[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br> | |||
[[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br> | |||
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | |||
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br> | |||
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br> | |||
[[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br> | |||
Revision as of 12:43, 24 February 2010
Project Information
29071-O99 rep Protein, CA42 rep Gene, Pre-Pro sequence
Construction Files
Jenna Kelleher 099 Rep Protein (sbb29)
Jenna Kelleher CA42 Rep Gene (sbb28)
Jenna Kelleher Pre-pro Sequence (sbb42)
Useful Protocols
For SBB28 and SBB29:
Cloning by PCR
Regular Zymo Cleanup
For SBB42:
Eco-Bam Transfer
Small-Frag Zymo Cleanup
For All 3:
EcoRI/BamHI Digest of PCR Products
Zymo Gel Purification
Ligation of EcoRI/BamHI digests
Transformation by heat-shock
Picking of colonies
Macherey-Nagel Miniprep
EcoRI/BamHI Digest of PCR Products
Analytical digests (Mapping)
