SBB09 protocols: Difference between revisions
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JCAnderson (talk | contribs) (New page: ===PCR Reactions=== {{Template:SBB-Protocols_PCR1 | Wobble PCR}}<br> {{Template:SBB-Protocols_PCR2 | Cloning by PCR}}<br> {{Template:SBB-Protocols_PCR3 | SOEing PCR}}<br> ===PCR Reactions...) |
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=== | __NOTOC__ | ||
[[SBB-Protocols_all | All these protocols in one big list]]<br> | |||
===Polymerase Reactions=== | |||
[[Template:SBB-Protocols_PCR1 | Wobble Reaction]]<br> | |||
[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | |||
[[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> | |||
=== | ===Cleanup Reactions=== | ||
[[Template:SBB-Protocols_Zymo1 | Regular Zymo Cleanup]]<br> | |||
[[Template:SBB-Protocols_Zymo2 | Small-Frag Zymo Cleanup]]<br> | |||
[[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br> | |||
=== | ===Digestions Reactions=== | ||
[[Template:SBB-Protocols_Enz1 | EcoRI/BamHI Digest of Wobble Products]]<br> | |||
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br> | |||
[[Template:SBB-Protocols_Enz3 | BglII Digest of EIPCR Products]]<br> | |||
[[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br> | |||
[[Template:SBB-Protocols_Enz5 | Ligation of EIPCR digests]]<br> | |||
[[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br> | |||
===Bacteria Manipulations=== | |||
[[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br> | |||
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | |||
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | |||
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br> | |||
===Assays=== | |||
[[Template:SBB-Protocols_Assay1 | Growth rate determination]]<br> | |||
[[Template:SBB-Protocols_Assay2 | Streptavidin-binding assay]]<br> | |||
[[Template:SBB-Protocols_Assay3 | Silver biomineralization assay]]<br> | |||
[[Template:SBB-Protocols_Assay4 | Cell-cell adhesion assay]]<br> | |||
===Other=== | |||
[[Template:SBB-Protocols_LRGtw | LR Gateway Transfers]]<br> | |||
[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | |||
Latest revision as of 17:42, 5 June 2009
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep
Assays
Growth rate determination
Streptavidin-binding assay
Silver biomineralization assay
Cell-cell adhesion assay
