SBB09 protocols: Difference between revisions
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[[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br> | [[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br> | ||
[[Template:SBB-Protocols_Enz5 | Ligation of EIPCR digests]]<br> | [[Template:SBB-Protocols_Enz5 | Ligation of EIPCR digests]]<br> | ||
[[Template:SBB-Protocols_Enz6 | Analytical digests]]<br> | [[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br> | ||
===Bacteria Manipulations=== | ===Bacteria Manipulations=== |
Revision as of 12:17, 2 March 2009
All these protocols in one big list
Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification
Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)
Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA