SBB09 protocols: Difference between revisions

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[[SBB-Protocols_all | All these protocols in one big list]]<br>
[[SBB-Protocols_all | All these protocols in one big list]]<br>
===PCR Reactions===
===PCR Reactions===
{{Template:SBB-Protocols_PCR1}}     Wobble PCR <br>
[[Template:SBB-Protocols_PCR1 | Wobble PCR]]<br>
{{Template:SBB-Protocols_PCR2}}     Cloning by PCR <br>
[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br>
{{Template:SBB-Protocols_PCR3}}     SOEing PCR <br>
[[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br>
   
 
===Cleanup Reactions===    
===Cleanup Reactions===
{{Template:SBB-Protocols_Zymo1}}     Regular Zymo Cleanup <br>
[[Template:SBB-Protocols_Zymo1 | Regular Zymo Cleanup]]<br>
{{Template:SBB-Protocols_Zymo2}}     Small-Frag Zymo Cleanup <br>
[[Template:SBB-Protocols_Zymo2 | Small-Frag Zymo Cleanup]]<br>
{{Template:SBB-Protocols_Zymo3}}     Zymo Gel Purification Cleanup <br>
[[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification Cleanup]]<br>
   
 
===Digestions Reactions===    
===Digestions Reactions===
{{Template:SBB-Protocols_Enz1}}     EcoRI/BamHI Digest of Wobble Products <br>
[[Template:SBB-Protocols_Enz1 | EcoRI/BamHI Digest of Wobble Products]]<br>
{{Template:SBB-Protocols_Enz2}}     EcoRI/BamHI Digest of PCR Products <br>
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br>
{{Template:SBB-Protocols_Enz3}}     BglII Digest of EIPCR Products <br>
[[Template:SBB-Protocols_Enz3 | BglII Digest of EIPCR Products]]<br>
{{Template:SBB-Protocols_Enz4}}     Ligation of EcoRI/BamHI digests <br>
[[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br>
{{Template:SBB-Protocols_Enz5}}     Ligation of EIPCR digests <br>
[[Template:SBB-Protocols_Enz5 | Ligation of EIPCR digests]]<br>
   
 
===Bacteria Manipulations===    
===Bacteria Manipulations===
{{Template:SBB-Protocols_Micro1}}     Transformation by heat-shock <br>
[[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br>
{{Template:SBB-Protocols_Micro2}}     Picking of colonies <br>
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br>
{{Template:SBB-Protocols_Micro3}}     Miniprep purification of DNA <br>
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br>

Revision as of 21:19, 3 February 2009

All these protocols in one big list

PCR Reactions

Wobble PCR
Cloning by PCR
SOEing PCR

Cleanup Reactions

Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification Cleanup

Digestions Reactions

EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests

Bacteria Manipulations

Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA

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