SBB09 Oligos: Difference between revisions
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Put your oligo sequences into this page. Separate each column with tabs: | Put your oligo sequences into this page. Separate each column with tabs: | ||
<pre> | <pre> | ||
Ost001F Forward oligo for upaG ccaaaGAATTCatgAGATCTgttgagatggataacaaactg | Ost001F Forward oligo for upaG ccaaaGAATTCatgAGATCTgttgagatggataacaaactg | ||
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Ost007F Forward construction of Magnetic Bead BP basic part ccaaaGAATTCatgAGATCTCACCACAAATACTGGCACCG | Ost007F Forward construction of Magnetic Bead BP basic part ccaaaGAATTCatgAGATCTCACCACAAATACTGGCACCG | ||
Ost008R Reverse construction of Magnetic Bead BP basic part ctagcGGATCCACGGTGCCAGTATTTGTGGTG | Ost008R Reverse construction of Magnetic Bead BP basic part ctagcGGATCCACGGTGCCAGTATTTGTGGTG | ||
Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg | |||
Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg | |||
Oso003 Cloning of PL2_Passenger_AT to Autotransporter Ag43 ccataGAATTCatgAGATCTacgctgacgctgaacgacag | |||
Oso001 Cloning of | Ojb001F Forward construction of CPG_L2 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg | ||
Oso002 Cloning of | Ojb001R Reverse construction of CPG_L2 N term part catgtGGATCCttacgcgctataatctaccggcc | ||
Oso003 Cloning of PL2_Passenger_AT to Autotransporter | Ojb002F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTggtaaacgtggaacgtggtt | ||
Ojb003F Forward removal of XhoI site in CPG_L2 actattatctTgagcgcggc | |||
Ojb003R Reverse removal of XhoI site in CPG_L2 gccgcgctcAagataatagt | |||
Ojb004F Forward SOEing oligo for CPG_L2 GGATCTAAGTCTCGTCGCgaggaaaggaacgactggca | |||
Ojb004R Reverse SOEing oligo for CPG_L2 GCGACGAGACTTAGATCCgaacgagtaatttacgccga | |||
Ojb001F | Ojb005F Forward construction of CPG_L6 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg | ||
Ojb001R | Ojb005R Reverse construction of CPG_L6 N term part catgtGGATCCttactgccagtcccagttactcc | ||
Ojb002F | Ojb006F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTgatgatattgaacgtgaagg | ||
Ojb003F | Ojb002R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga | ||
Ojb003R | Bhs001F Forward EcoRI for a~eaeA_Display> cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG | ||
Bhs002F Removing the EcoRI site from eaeA_Display GTTAATCAGAACTCATTTGCAAATGG | |||
Ojb004F | Bhs002R Removing the EcoRI site from eaeA_Display CCATTTGCAAATGAGTTCTGATTAAC | ||
Ojb004R | Bhs003R Reverse BamHI for a~eaeA_Display> GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC | ||
Ojb005F | Osn0001-F Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai ccagtGAATTCatgAGATCTgccccgtcagcatctgccac | ||
Ojb005R | Osn0002-R Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai gcagtGGATCCtcagaacgagtaacggaaattag | ||
Ojb006F | |||
Bhs001F | |||
Bhs002F | |||
Bhs002R | |||
Bhs003R | |||
Osn0001-F | |||
Osn0002-R | |||
Odd001F Forward EcoRI oligo for Tsh Autotransporter ccataGAATTCatgAGATCTacacttttacctgtatacg | Odd001F Forward EcoRI oligo for Tsh Autotransporter ccataGAATTCatgAGATCTacacttttacctgtatacg | ||
Odd002R Reverse BamHI oligo for Tsh Autotransporter ctgatGGATCCtcagaatgaataacgaatattagcg | Odd002R Reverse BamHI oligo for Tsh Autotransporter ctgatGGATCCtcagaatgaataacgaatattagcg | ||
Odd003F Forward oligo for Ice Nucleation Protein ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg | Odd003F Forward oligo for Ice Nucleation Protein ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg | ||
Odmd001F Forward Oligo for Invasin (refactored, long) ccataGAATTCatgAGATCTGCTGGGGCTTCAGAAAAATATGATGC | |||
Odmd002R Reverse Oligo for Invasin (refactored, long) ccaaaGGATCCactggataccagcgatctgccg | |||
Ocr0001-F forward oligo for biobricking of {a~Inv-short>} ccaaaGAATTCatgAGATCTAATTATGATGGTTTTCCAGC | |||
Ocr0001-R reverse oligo for biobricking of {a~Inv-short>} ccaaaGGATCCactggataccagcgatctgccgc | |||
Odmd001F | Odmd003R Reverse Oligo for Chal Lectin w/o Stop Codon ccaaaGGATCCctcgctgtcgtcctctcgta | ||
Odmd002R | Ons003F Forward construction of Poly_bind basic part ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg | ||
Odmd003R | Ons004R Reverse construction of Poly_bind basic part ctgatGGATCCcccccgtattacatgttcgtatagccagaat | ||
< | Ons001F Forward oligo for TraT ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc | ||
Ons002R Reverse oligo for TraT ctgatGGATCCgagaatatttgcgattgatttgg | |||
< | Odvs003 Gene Synthesis of CBK basic part CCATAGAATTCATGAGATCTGGTTCTGGTCCGACCCAGTCTCACTACGGTCAGTGCG | ||
Odvs0007F | Odvs004 Gene Synthesis of CBK basic part CGCAAACGGTCGGACCAGAGTAACCGATACCACCGCACTGACCGTAGTGAGACTG | ||
Odvs0007R | Odvs005 Gene Synthesis of CBK basic part CTCTGGTCCGACCGTTTGCGCTTCTGGTACCACCTGCCAGGTTCTGAACCCGTACTAC | ||
Odvs0008rF | Odvs006 Gene Synthesis of CBK basic part GCTTAGGATCCACCAGAACCCAGGCACTGAGAGTAGTACGGGTTCAGAACCTGGC | ||
Odvs0008rR | OWRL-025-F Forward construction of Aluminum Binding Peptide basic part GCGTAgaattcATGagatctGTGCCGAGCAGCGGCCCGCAGGATACC | ||
< | OWRL-026-R Reverse construction of Aluminum Binding Peptide TCATCggatccGGTGGTGCGGGTATCCTGCGGGCCGCTGC | ||
Oph022F Foward oligo for cloning of {a~TraA>} ccaaaGAATTCatgAGATCTacttatgaatgctgttttaagtgttcaggg | |||
< | Oph023R Reverse oligo for removal of stop codon {a~TraA>} gcaaaGGATCCgaggccaacgacggccatac | ||
Osc0030F | js001F Forward biobricking of upaG (long) CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg | ||
Osc0031R | js002R Reverse biobricking of upaG (long) CTGATggatccTTACCACtgaataccggcac | ||
Oeh001F Forward EcoRI/BglII oligo for <INP repeats> gtcagGAATTCatgAGATCTaaccacagtgatctaattgc | |||
Oeh001R Reverse BamHI oligo for <INP repeats> gtcagGGATCCactgtcctgcccggccgttg | |||
</ | Odvs002F Forward construction of AG4 basic part ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC | ||
Odvs002R Reverse construction of AG4 basic part ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG | |||
OWRL-023-F Forward biobricking of Intimin EaeA GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG | |||
Odvs0007F Forward EcoRI/BglII for Bglbrick of ECOHLY ccataGAATTCATGAGATCTtattccgtggaagaacttattgggacc | |||
Odvs0007R Reverse BamHI for Bglbrick of ECOHLY ctgatGGATCCataaatatcattaccatatccacc | |||
Odvs0008rF Removing the BglII site in ECOHLY (Forward) ggcagtgagggagcagaCctgcttgatggcgg | |||
Odvs0008rR Removing the BglII site in ECOHLY (Reverse) ccgccatcaagcagGtctgctccctcactgcc | |||
Omv044F Forward Bglbricking of {<N.fliD>} ccagtGAATTCatgAGATCTgcaagtatttcatcgctggg | |||
Omv044R Reverse Bglbricking of {<N.fliD>} gcagtGGATCCttaaaaactttgtagcgcatcatcaccgc | |||
Omv045F Forward Removing BamHI site in {<N.fliD>} cgttaagcggCatccgtgatgcc | |||
Omv045R Reverse Removing BamHI site in {<N.fliD>} ggcatcacggatGccgcttaacg | |||
Omv046F Forward Bglbricking of {<C.fliD>} ccagtGAATTCatgAGATCTgttgccgcccagaatgcg | |||
Omv046R Reverse Bglbricking of {<C.fliD>} gcagtGGATCCcttggaattactgttgttttcg | |||
Ovu015 Construction of cpx N term part ctagaGAATTCatgAGATCTGGTCAGTCTggtgactacaacaaaaaccag | |||
Ovu016 Construction of cpx N term part gacaaGGATCCgaagcggtaaccaacagaggcaatccaggtgcctac | |||
Ovu017 Construction of cpx C term part ctagaGAATTCatgAGATCTgcgacttctactgtaactgg | |||
Ovu018 Construction of cpx C term part gacaaGGATCCttaagagcttgcagtacggcttttctcgg | |||
Ovu019 SOEing of eCPX gtcgcTTTAGACTGTTTAGATCCgaagcggtaaccaacag | |||
Ovu020 SOEing of eCPX GGATCTAAACAGTCTAAAgcgacttctactgtaactgg | |||
Osc0030F Forward Biobricking of ehaB ggcctGAATTCattAGATCTggcttaatcgcaatgaactct | |||
Osc0031R Reverse Biobricking of ehaB gtaccGGATCCttaccaggtatatttaacac | |||
Omv047F Forward construction of {<linker1>} basic part ccataGAATTCatgAGATCTtacttcgatgtctggggcgcagggaccacggtcaccgtctcctcaggcggag | |||
Omv047R Reverse construction of {<linker1>} basic part ctgatGGATCCtcctcctccagaaccacctccacctgaaccgcctccgcctgaggagacggtgac | |||
Omv048F Forward construction of {<linker2>} basic part ccataGAATTCatgAGATCTtggacgttcggtggaggcaccaagctggaaatcaaacgtgcggccgctc | |||
Omv048R Reverse construction of {<linker2>} basic part ctgatGGATCCacggccttcaatgccgtgatggtgatggtggtgagcggccgcacgtttgatttc | |||
Ojw001 Forward construction of Z34C basic part CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG | |||
Ojw002 Reverse construction of Z34C basic part CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG | |||
Oph024F Foward oligo for cloning of {<KILR>} ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg | |||
Oph025R Reverse oligo for cloning of {<KILR>} ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc | |||
Osc0028F Forward construction of Leucine zipper EILD TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG | |||
Osc0029R Reverse construction of Leucine zipper EILD CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG | |||
</Pre> |
Latest revision as of 22:53, 12 February 2009
Put your oligo sequences into this page. Separate each column with tabs:
Ost001F Forward oligo for upaG ccaaaGAATTCatgAGATCTgttgagatggataacaaactg Ost002R Reverse oligo for upaG ctagcGGATCCttaCCACtgaataccggcaccgag Ost003F Forward construction for HydroxyapatiteBP ccaaaGAATTCatgAGATCTgcgccgtggcatctgagcagccagtatag Ost004R Reverse construction for HydroxyapatiteBP ctagcGGATCCggtgcggctatactggctgctcagatgc Ost005F Forward oligo for Beta Helix Repeats ccaaaGAATTCatgAGATCTagtgtcttcaacggcaccg Ost006R Reverse oligo for Beta Helix Repeats ctagcGGATCCtgtacgcatgacaaatgctgac Ost007F Forward construction of Magnetic Bead BP basic part ccaaaGAATTCatgAGATCTCACCACAAATACTGGCACCG Ost008R Reverse construction of Magnetic Bead BP basic part ctagcGGATCCACGGTGCCAGTATTTGTGGTG Oso001 Cloning of Ag43, short ccataGAATTCatgAGATCTggtgaaaacaacagcgtccg Oso002 Cloning of Ag43 GCttaggatcctcagaaggtcacattcagtg Oso003 Cloning of PL2_Passenger_AT to Autotransporter Ag43 ccataGAATTCatgAGATCTacgctgacgctgaacgacag Ojb001F Forward construction of CPG_L2 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb001R Reverse construction of CPG_L2 N term part catgtGGATCCttacgcgctataatctaccggcc Ojb002F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTggtaaacgtggaacgtggtt Ojb003F Forward removal of XhoI site in CPG_L2 actattatctTgagcgcggc Ojb003R Reverse removal of XhoI site in CPG_L2 gccgcgctcAagataatagt Ojb004F Forward SOEing oligo for CPG_L2 GGATCTAAGTCTCGTCGCgaggaaaggaacgactggca Ojb004R Reverse SOEing oligo for CPG_L2 GCGACGAGACTTAGATCCgaacgagtaatttacgccga Ojb005F Forward construction of CPG_L6 N term part ctctgGAATTCatgAGATCTgaggaaaggaacgactgg Ojb005R Reverse construction of CPG_L6 N term part catgtGGATCCttactgccagtcccagttactcc Ojb006F Forward construction of CPG_L2 C term part ctctgGAATTCatgAGATCTgatgatattgaacgtgaagg Ojb002R Reverse construction of CPG_L2 C term part catgtGGATCCgaacgagtaatttacgccga Bhs001F Forward EcoRI for a~eaeA_Display> cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG Bhs002F Removing the EcoRI site from eaeA_Display GTTAATCAGAACTCATTTGCAAATGG Bhs002R Removing the EcoRI site from eaeA_Display CCATTTGCAAATGAGTTCTGATTAAC Bhs003R Reverse BamHI for a~eaeA_Display> GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC Osn0001-F Forward Bglbricking of espP of Escherichia coli O157:H7 str. Sakai ccagtGAATTCatgAGATCTgccccgtcagcatctgccac Osn0002-R Reverse Bglbricking of espP of Escherichia coli O157:H7 str. Sakai gcagtGGATCCtcagaacgagtaacggaaattag Odd001F Forward EcoRI oligo for Tsh Autotransporter ccataGAATTCatgAGATCTacacttttacctgtatacg Odd002R Reverse BamHI oligo for Tsh Autotransporter ctgatGGATCCtcagaatgaataacgaatattagcg Odd003F Forward oligo for Ice Nucleation Protein ccaaaGAATTCatgAGATCTtgtaATGaatctcgacaaggcg Odmd001F Forward Oligo for Invasin (refactored, long) ccataGAATTCatgAGATCTGCTGGGGCTTCAGAAAAATATGATGC Odmd002R Reverse Oligo for Invasin (refactored, long) ccaaaGGATCCactggataccagcgatctgccg Ocr0001-F forward oligo for biobricking of {a~Inv-short>} ccaaaGAATTCatgAGATCTAATTATGATGGTTTTCCAGC Ocr0001-R reverse oligo for biobricking of {a~Inv-short>} ccaaaGGATCCactggataccagcgatctgccgc Odmd003R Reverse Oligo for Chal Lectin w/o Stop Codon ccaaaGGATCCctcgctgtcgtcctctcgta Ons003F Forward construction of Poly_bind basic part ccataGAATTCatgAGATCTttcaaattctggctatacgaacatg Ons004R Reverse construction of Poly_bind basic part ctgatGGATCCcccccgtattacatgttcgtatagccagaat Ons001F Forward oligo for TraT ccataGAATTCatgAGATCTtgtggtgcgatgagcacagc Ons002R Reverse oligo for TraT ctgatGGATCCgagaatatttgcgattgatttgg Odvs003 Gene Synthesis of CBK basic part CCATAGAATTCATGAGATCTGGTTCTGGTCCGACCCAGTCTCACTACGGTCAGTGCG Odvs004 Gene Synthesis of CBK basic part CGCAAACGGTCGGACCAGAGTAACCGATACCACCGCACTGACCGTAGTGAGACTG Odvs005 Gene Synthesis of CBK basic part CTCTGGTCCGACCGTTTGCGCTTCTGGTACCACCTGCCAGGTTCTGAACCCGTACTAC Odvs006 Gene Synthesis of CBK basic part GCTTAGGATCCACCAGAACCCAGGCACTGAGAGTAGTACGGGTTCAGAACCTGGC OWRL-025-F Forward construction of Aluminum Binding Peptide basic part GCGTAgaattcATGagatctGTGCCGAGCAGCGGCCCGCAGGATACC OWRL-026-R Reverse construction of Aluminum Binding Peptide TCATCggatccGGTGGTGCGGGTATCCTGCGGGCCGCTGC Oph022F Foward oligo for cloning of {a~TraA>} ccaaaGAATTCatgAGATCTacttatgaatgctgttttaagtgttcaggg Oph023R Reverse oligo for removal of stop codon {a~TraA>} gcaaaGGATCCgaggccaacgacggccatac js001F Forward biobricking of upaG (long) CCATAgaattcATGagatctcgcaaaattgttaatatggctgctg js002R Reverse biobricking of upaG (long) CTGATggatccTTACCACtgaataccggcac Oeh001F Forward EcoRI/BglII oligo for <INP repeats> gtcagGAATTCatgAGATCTaaccacagtgatctaattgc Oeh001R Reverse BamHI oligo for <INP repeats> gtcagGGATCCactgtcctgcccggccgttg Odvs002F Forward construction of AG4 basic part ccataGAATTCATGAGATCTAACCCGTCTTCTCTGTTCCGTTACCTGCC Odvs002R Reverse construction of AG4 basic part ctgatGGATCCGTCAGACGGCAGGTAACGGAACAGAGAAGACGG OWRL-023-F Forward biobricking of Intimin EaeA GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG Odvs0007F Forward EcoRI/BglII for Bglbrick of ECOHLY ccataGAATTCATGAGATCTtattccgtggaagaacttattgggacc Odvs0007R Reverse BamHI for Bglbrick of ECOHLY ctgatGGATCCataaatatcattaccatatccacc Odvs0008rF Removing the BglII site in ECOHLY (Forward) ggcagtgagggagcagaCctgcttgatggcgg Odvs0008rR Removing the BglII site in ECOHLY (Reverse) ccgccatcaagcagGtctgctccctcactgcc Omv044F Forward Bglbricking of {<N.fliD>} ccagtGAATTCatgAGATCTgcaagtatttcatcgctggg Omv044R Reverse Bglbricking of {<N.fliD>} gcagtGGATCCttaaaaactttgtagcgcatcatcaccgc Omv045F Forward Removing BamHI site in {<N.fliD>} cgttaagcggCatccgtgatgcc Omv045R Reverse Removing BamHI site in {<N.fliD>} ggcatcacggatGccgcttaacg Omv046F Forward Bglbricking of {<C.fliD>} ccagtGAATTCatgAGATCTgttgccgcccagaatgcg Omv046R Reverse Bglbricking of {<C.fliD>} gcagtGGATCCcttggaattactgttgttttcg Ovu015 Construction of cpx N term part ctagaGAATTCatgAGATCTGGTCAGTCTggtgactacaacaaaaaccag Ovu016 Construction of cpx N term part gacaaGGATCCgaagcggtaaccaacagaggcaatccaggtgcctac Ovu017 Construction of cpx C term part ctagaGAATTCatgAGATCTgcgacttctactgtaactgg Ovu018 Construction of cpx C term part gacaaGGATCCttaagagcttgcagtacggcttttctcgg Ovu019 SOEing of eCPX gtcgcTTTAGACTGTTTAGATCCgaagcggtaaccaacag Ovu020 SOEing of eCPX GGATCTAAACAGTCTAAAgcgacttctactgtaactgg Osc0030F Forward Biobricking of ehaB ggcctGAATTCattAGATCTggcttaatcgcaatgaactct Osc0031R Reverse Biobricking of ehaB gtaccGGATCCttaccaggtatatttaacac Omv047F Forward construction of {<linker1>} basic part ccataGAATTCatgAGATCTtacttcgatgtctggggcgcagggaccacggtcaccgtctcctcaggcggag Omv047R Reverse construction of {<linker1>} basic part ctgatGGATCCtcctcctccagaaccacctccacctgaaccgcctccgcctgaggagacggtgac Omv048F Forward construction of {<linker2>} basic part ccataGAATTCatgAGATCTtggacgttcggtggaggcaccaagctggaaatcaaacgtgcggccgctc Omv048R Reverse construction of {<linker2>} basic part ctgatGGATCCacggccttcaatgccgtgatggtgatggtggtgagcggccgcacgtttgatttc Ojw001 Forward construction of Z34C basic part CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG Ojw002 Reverse construction of Z34C basic part CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG Oph024F Foward oligo for cloning of {<KILR>} ccataGAATTCatgAGATCTgttaaagaactggaagacaaaaacgaagaactgctgtctaaaatctaccacctgcg Oph025R Reverse oligo for cloning of {<KILR>} ctgatGGATCCgcaaccaccacgttcaccaaccagttttttcagacgagcaacttcgttacgcaggtggtagattttagacagc Osc0028F Forward construction of Leucine zipper EILD TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG Osc0029R Reverse construction of Leucine zipper EILD CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG