SBB09 Construct5: Difference between revisions
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<pre> | <pre> | ||
Construction of {a~ | Construction of {a~Inv-native>} basic part | ||
PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (145 bp, gp = A) | PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (145 bp, gp = A) | ||
PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1888 bp, gp = B) | PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1888 bp, gp = B) | ||
| Line 6: | Line 6: | ||
PCR Bhs001F/Bhs003R on A+B (2008 bp, EcoRI/BamHI) | PCR Bhs001F/Bhs003R on A+B (2008 bp, EcoRI/BamHI) | ||
Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) | Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) | ||
Product is pBca9495CA-M10024 {a~ | Product is pBca9495CA-M10024 {a~Inv-native>} | ||
---------------------------- | ---------------------------- | ||
Bhs001F Forward EcoRI for a~ | Bhs001F Forward EcoRI for {a~Inv-native>} cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG | ||
Bhs002F Removing the EcoRI site from | Bhs002F Removing the EcoRI site from {a~Inv-native>} GTTAATCAGAAcTCATTTGCAAATG | ||
Bhs002R Removing the EcoRI site from | Bhs002R Removing the EcoRI site from {a~Inv-native>} CATTTGCAAATGAgTTCTGATTAAC | ||
Bhs003R Reverse BamHI for a~ | Bhs003R Reverse BamHI for {a~Inv-native>} GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC | ||
</pre> | </pre> | ||
JCA: There is a frame error on Bhs003R. Revised: | JCA: There is a frame error on Bhs003R. Revised: | ||
<pre> | <pre> | ||
Construction of {a~ | Construction of {a~Inv-native>} basic part | ||
PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (146 bp, gp = A) | PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (146 bp, gp = A) | ||
PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1889 bp, gp = B) | PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1889 bp, gp = B) | ||
| Line 22: | Line 22: | ||
PCR Bhs001F/Bhs003R on A+B (2009 bp, EcoRI/BamHI) | PCR Bhs001F/Bhs003R on A+B (2009 bp, EcoRI/BamHI) | ||
Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) | Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) | ||
Product is pBca9495CA-M10024 {a~ | Product is pBca9495CA-M10024 {a~Inv-native>} | ||
---------------------------- | ---------------------------- | ||
Bhs001F Forward EcoRI for a~ | Bhs001F Forward EcoRI for {a~Inv-native>} cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG | ||
Bhs002F Removing the EcoRI site from | Bhs002F Removing the EcoRI site from {a~Inv-native>} GTTAATCAGAACTCATTTGCAAATGG | ||
Bhs002R Removing the EcoRI site from | Bhs002R Removing the EcoRI site from {a~Inv-native>} CCATTTGCAAATGAGTTCTGATTAAC | ||
Bhs003R Reverse BamHI for a~ | Bhs003R Reverse BamHI for {a~Inv-native>} GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC | ||
</pre> | </pre> | ||
Revision as of 19:15, 9 May 2009
Construction of {a~Inv-native>} basic part
PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (145 bp, gp = A)
PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1888 bp, gp = B)
----------------------------
PCR Bhs001F/Bhs003R on A+B (2008 bp, EcoRI/BamHI)
Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10024 {a~Inv-native>}
----------------------------
Bhs001F Forward EcoRI for {a~Inv-native>} cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG
Bhs002F Removing the EcoRI site from {a~Inv-native>} GTTAATCAGAAcTCATTTGCAAATG
Bhs002R Removing the EcoRI site from {a~Inv-native>} CATTTGCAAATGAgTTCTGATTAAC
Bhs003R Reverse BamHI for {a~Inv-native>} GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC
JCA: There is a frame error on Bhs003R. Revised:
Construction of {a~Inv-native>} basic part
PCR Bhs001F/Bhs002R on E. coli strain 0157:H7 (146 bp, gp = A)
PCR Bhs002F/Bhs003R on E. coli strain 0157:H7 (1889 bp, gp = B)
----------------------------
PCR Bhs001F/Bhs003R on A+B (2009 bp, EcoRI/BamHI)
Digest pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L)
Product is pBca9495CA-M10024 {a~Inv-native>}
----------------------------
Bhs001F Forward EcoRI for {a~Inv-native>} cccaaGAATTCatgAGATCTtaacATGATTACTCATGGTTG
Bhs002F Removing the EcoRI site from {a~Inv-native>} GTTAATCAGAACTCATTTGCAAATGG
Bhs002R Removing the EcoRI site from {a~Inv-native>} CCATTTGCAAATGAGTTCTGATTAAC
Bhs003R Reverse BamHI for {a~Inv-native>} GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC
