SBB09 Construct46

Construction of mini protein A Z domain mutant

Wobble Ojw001 / Ojw002              (133bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144#5         (EcoRI/BamHI, L)
Product is pBca9495AK-M10018          {<Z34C domain>}
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Ojw001 Forward construction of Z34C basic part
CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG
Ojw002 Reverse construction of Z34C basic part
CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG