SBB09 Construct41: Difference between revisions
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PCR mea036/mea037 on pBca9145-Bca1126 (1219 bp, EcoRI/BamHI) | PCR mea036/mea037 on pBca9145-Bca1126 (1219 bp, EcoRI/BamHI) | ||
Sub into pBca9495AK-Bca1144 (EcoRI/BamHI, 3171+910, L) | Sub into pBca9495AK-Bca1144 (EcoRI/BamHI, 3171+910, L) | ||
Product is pBca9495AK- | Product is pBca9495AK-M10055 {<phoA>} | ||
---------------------------------------- | ---------------------------------------- | ||
mea036 Forward Biobricking of {<phoA>} cgatagaattcatgAGATCTGGAGATTCTG | mea036 Forward Biobricking of {<phoA>} cgatagaattcatgAGATCTGGAGATTCTG | ||
mea037 Reverse Biobricking of {<phoA>} cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG | mea037 Reverse Biobricking of {<phoA>} cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG | ||
</pre> | </pre> | ||
Latest revision as of 00:50, 10 February 2009
Construction of Alkaline Phosphatease Basic Part
PCR mea036/mea037 on pBca9145-Bca1126 (1219 bp, EcoRI/BamHI)
Sub into pBca9495AK-Bca1144 (EcoRI/BamHI, 3171+910, L)
Product is pBca9495AK-M10055 {<phoA>}
----------------------------------------
mea036 Forward Biobricking of {<phoA>} cgatagaattcatgAGATCTGGAGATTCTG
mea037 Reverse Biobricking of {<phoA>} cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG
